文献类型：期刊文献

英文题名：Effects of drought and salt-stresses on gene expression in Caragana korshinskii seedlings revealed by RNA-seq

作者：Li, Shaofeng[1] Fan, Chengming[2] Li, Yan[2] Zhang, Jianhui[2] Sun, Jingshuang[1] Chen, Yuhong[2] Tian, Changyan[3] Su, Xiaohua[4] Lu, Mengzhu[4] Liang, Chengzhi[2] Hu, Zanmin[2]

通信作者：Hu, ZM[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 100023, Peoples R China;[2]Chinese Acad Sci, Inst Genet & Dev Biol, Datun Rd, Beijing 100101, Peoples R China;[3]Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2016

卷号：17

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000371595700005)】；

基金：We thank Dr. Chaoying He (Institute of Botany, Chinese Academy of Sciences) for the helpful advice and technical assistance. We also thank Dr. Liying Song (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) and Dr. Jin Xu and Jue Ruan (Beijing Institute of Genomics, Chinese Academy of Sciences) for their sequencing work. This work was supported by a project (2011AA10020105) at the Ministry of Science and Technology of China and a project from National Natural Science Foundation of China (31400570).

语种：英文

外文关键词：Caragana korshinskii; Transcriptome; Illumina sequencing; Drought-stress tolerance; Salt-stress tolerance

摘要：Background: Drought and soil salinity are major abiotic stresses. The mechanisms of stress tolerance have been studied extensively in model plants. Caragana korshinskii is characterized by high drought and salt tolerance in northwestern China; unique patterns of gene expression allow it to tolerate the stress imposed by dehydration and semi-desert saline soil. There have, however, been no reports on the differences between C. korshinskii and model plants in the mechanisms underlying their drought and salt tolerance and regulation of gene expression. Results: Three sequencing libraries from drought and salt-treated whole-seedling-plants and the control were sequenced to investigate changes in the C. korshinskii transcriptome in response to drought and salt stresses. Of the 129,451 contigs, 70,662 (54.12 %) were annotated with gene descriptions, gene ontology (GO) terms, and metabolic pathways, with a cut-off E-value of 10(-5). These annotations included 56 GO terms, 148 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and 25 Clusters of Orthologous Groups (COG). On comparison of the transcriptomes of the control, drought-and salt-treated plants, 1630 and 1521 contigs showed significant differences in transcript abundance under drought and salt stresses. Compared to the differentially expressed genes (DEGs) in drought-or salt-treated Arabidopsis in the database, 542 DEGs in drought-treated C. korshinskii and 529 DEGs in salt-treated samples were presumably unique to C. korshinskii. The transcription profiles revealed that genes related to transcription factors, protein kinases, and antioxidant enzymes are relevant to the tolerance of drought and salt stress in this species. The expression patterns of 38 randomly selected DEGs were confirmed by quantitative real-time PCR and were essentially consistent with the changes in transcript abundance identified by RNA-seq. Conclusions: The present study identified potential genes involved in drought and salt tolerance in C. korshinskii, as well as many DEGs uniquely expressed in drought-or salt-treated C. korshinskii samples compared to Arabidopsis. To our knowledge, this study is the first exploration of the C. korshinskii transcriptome under drought and salt conditions, and these results will facilitate the discovery of specific stress-resistance-related genes in C. korshinskii, possibly leading to the development of novel plant cultivars through genetic engineering.