文献类型：期刊文献

中文题名：两步生根法中4种因素对尾巨桉组培苗生根的影响

英文题名：Effect of Four Factors in Two-step Rooting Method on Rooting of Eucalyptus urophylla×E.grandis Clones

作者：黄安瀛[1] 罗建中[1] 陈铭秋[1,2] 卢万鸿[1] 燕青[3] 林彦[1]

第一作者：黄安瀛

机构：[1]中国林业科学研究院速生树木研究所,广东湛江524300;[2]西南林业大学,昆明650224;[3]中国热带农业科学院湛江实验站,广东湛江524013

年份：2025

卷号：33

期号：4

起止页码：399-406

中文期刊名：热带亚热带植物学报

外文期刊名：Journal of Tropical and Subtropical Botany

收录：;北大核心:【北大核心2023】；

基金：“十四五”国家重点研发计划项目(2022YFD2200203-3);广东省林业科技创新项目(221KJCX019)资助。

语种：中文

中文关键词：尾巨桉;组培;无性系;生根率;根系形态

外文关键词：Eucalyptus urophylla×E.grandis;Tissue culture;Clone;Rooting rate;Root morphology

分类号：S792.39

摘要：组培苗的生根能力直接关系到其后续移栽成活率和生长能力,桉树组培苗的生根研究对提升苗木生根质量、降低成本有着重要意义。以来源于同一杂交组合的4个尾巨桉(Eucalyptus urophylla×E.grandis)无性系(EC262、EC264、EC269和EC272)为试验材料,设计4因素(暗培养时长、培养基1、培养基2、无性系)4水平正交试验,接种后培养30 d时测量其生根相关指标,并利用极差分析对数据进行处理评价。结果表明,不同处理下的无性系生根率、根数、平均根长、生根性状评分率、愈伤率等生根性状均在P<0.05水平表现出显著差异。生根率为12.5%~65%;平均生根量为1.9~3.57条;平均根长为2.55~4.81 cm;生根性状评分率为28%~78%;愈伤率为10%~65%。极差分析表明诱导阶段的培养基(含生长调节剂)对平均根长的影响最大,极差为4.19;生根性状则主要受根生长阶段的培养基影响,其极差为1.02,无性系为影响愈伤率的最重要因素,极差为8.5。因此,4个尾巨桉无性系生根阶段平均根长受诱导阶段的环境因素影响更大,生根性状则受到生长阶段环境因素影响更大,此外,虽然遗传因素(无性系)与环境因素(暗环境、培养基)均对愈伤率有显著影响,但遗传因素影响更大。
The rooting ability of tissue culture seedlings is directly related to their subsequent transplanting survival rate and growth capacity.The study on the rooting of Eucalyptus urophylla×E.grandis tissue culture seedlings is of great significance for improving the quality of seedling rooting and reducing costs.Four E.urophylla×E.grandis clones(EC262,EC264,EC269 and EC272)from the same hybrid combination were used as experimental materials.A 4-factor(dark culture duration,medium 1,medium 2,clone)4-level orthogonal experiment was designed.The rooting-related indicators were measured 30 days after inoculation,and the data were processed and evaluated by range analysis.The results showed that the rooting rate,number of roots,average root length,rooting trait score rate and callus rate of the clones under different treatments were significantly different at 0.05 level.The rooting rate ranged from 12.5%to 65%;the average number of roots was 1.9 to 3.57;the average root length ranged from 2.55 to 4.81 cm;the rooting trait score rate was 28%to 78%;and the callus rate was 10%to 65%.Range analysis indicated that the medium(containing growth regulators)in the induction stage had the greatest effect on the average root length,with a range of 4.19;the rooting traits were mainly affected by the medium in the root growth stage,with a range of 1.02;and the clone was the most important factor affecting the callus rate,with a range of 8.5.Therefore,the average root length of the four E.urophylla×E.grandis clones during the rooting stage was more affected by the environmental factors in the induction stage,while the rooting traits were more affected by the environmental factors in the growth stage.In addition,although both genetic factors(clones)and environmental factors(dark environment,medium)had significant effects on the callus rate,the genetic factors had a greater influence.