文献类型：期刊文献

英文题名：Comparative analysis of the Dicer-like gene family reveals loss of miR162 target site in SmDCL1 from Salvia miltiorrhiza

作者：Shao, Fenjuan[1,2,3] Qiu, Deyou[3] Lu, Shanfa[1,2]

第一作者：Shao, Fenjuan;邵芬娟

通信作者：Lu, SF[1]

机构：[1]Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100730, Peoples R China;[2]Peking Union Med Coll, Beijing 100021, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2015

卷号：5

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000355215500001)】；

基金：We thank Dr. Shilin Chen and the S. miltiorrhiza sequencing group for kindly providing the S. miltiorrhiza genome sequence. We appreciate Prof. Xian'en Li for providing S. miltiorrhiza plants. This work was supported by grants from the Natural Science Foundation of China (grant no. 31370327), the Beijing Natural Science Foundation (grant nos. 5112026 and 5152021), the Major Scientific and Technological Special Project for Significant New Drugs Creation (grant no. 2012ZX09301002-001-031), the Research Fund for the Doctoral Program of Higher Education of China (20111106110033), the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT, grant no. IRT1150), and the Fund for Postgraduate Innovation in Peking Union Medical College (2012-1007-026).

语种：英文

摘要：DCL1, the core component for miRNA biogenesis, is itself regulated by miR162 in Arabidopsis. MiRNA-mediated feedback regulation of AtDCL1 is important to maintain the proper level of DCL1 transcripts. However, it is unknown whether the miRNA-mediated regulation of DCL1 is conserved among plants. We analyzed the SmDCL gene family in Salvia miltiorrhiza, an emerging model plant for Traditional Chinese Medicine (TCM) studies, using a comprehensive approach integrating genome-wide prediction, molecular cloning, gene expression profiling, and posttranscriptional regulation analysis. A total of five SmDCLs were identified. Comparative analysis of SmDCLs and AtDCLs showed an apparent enlargement of SmDCL introns in S. miltiorrhiza. The absence of miR162 in S. miltiorrhiza and the loss of miR162 target site in SmDCL1 were unexpectedly found. Further analysis showed that the miR162 target site was not present in DCL1 from ancient plants and was gained during plant evolution. The gained miR162 target site might be lost in a few modern plants through nucleotide mutations. Our results provide evidence for the gain and loss of miR162 and its target sites in Dicer-like genes during evolution. The data is useful for understanding the evolution of miRNA-mediated feedback regulation of DCLs in plants.