文献类型：期刊文献

英文题名：A method for analyzing programmed cell death in xylem development by flow cytometry

作者：Liu, Ying-Li[1] Guo, Ying-Hua[2] Song, Xue-Qin[3] Hu, Meng-Xuan[3] Zhao, Shu-Tang[3]

第一作者：Liu, Ying-Li

通信作者：Zhao, ST[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Peking Univ, Natl Ctr Prot Sci, Beijing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85163643196);WOS:【SCI-EXPANDED(收录号:WOS:001016200200001)】；

基金：This work was supported by the National Key Research and Development Program of China (2019YFE0119100).

语种：英文

外文关键词：PCD; xylem development; FACS; gene expression; woody plants

摘要：Programmed cell death (PCD) is a genetically regulated developmental process leading to the death of specific types of plant cells, which plays important roles in plant development and growth such as wood formation. However, an efficient method needs to be established to study PCD in woody plants. Flow cytometry is widely utilized to evaluate apoptosis in mammalian cells, while it is rarely used to detect PCD in plants, especially in woody plants. Here, we reported that the xylem cell protoplasts from poplar stem were stained with a combination of fluorescein annexin V-FITC and propidium iodide (PI) and then sorted by flow cytometry. As expected, living cells (annexin V-FITC negative/PI negative), early PCD cells (annexin V-FITC positive/PI negative), and late PCD cells (annexin V-FITC positive/PI positive) could be finely distinguished through this method and then subjected for quantitative analysis. The expression of cell-type- and developmental stages-specific marker genes was consistent with the cell morphological observation. Therefore, the newly developed fluorescence-activated cell sorting (FACS) method can be used to study PCD in woody plants, which will be beneficial for studying the molecular mechanisms of wood formation.