文献类型：期刊文献

中文题名：9个中国选育油橄榄品种母系来源多样性研究

英文题名：Maternal origin diversity of nine olive varieties bred in China

作者：马宏博[1] 王桢莹[1] 赵海云[2] 张建国[1] 曾艳飞[1]

第一作者：马宏博

机构：[1]中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,北京100091;[2]陇南市武都区油橄榄产业开发办公室,甘肃陇南746000

年份：2024

卷号：44

期号：11

起止页码：1760-1768

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：“十四五”国家重点研发计划项目(2023YFD2200301)。

语种：中文

中文关键词：油橄榄;叶绿体基因组;品种选育;系统发育;母系来源

外文关键词：olive(Olea europaea L.);chloroplast genome;variety selected and bred;phylogeny;mater-nal origin

分类号：Q941.2;S565.7

摘要：【目的】比较中国选育油橄榄品种与地中海油橄榄叶绿体基因组的多样性和亲缘关系,评估中国选育品种母系来源多样性,为中国油橄榄种质开发提供依据。【方法】基于本研究所测的9个中国选育油橄榄品种、7个伊朗种质和下载的52个地中海油橄榄种质的重测序数据,用NOVOPlasty组装叶绿体基因组,计算序列多态性;用GPA和RAxML-NG、Network 5.0等软件分别构建叶绿体基因组图谱、系统发育和单倍型网络关系。【结果】油橄榄叶绿体基因组在其四分体结构、基因数量上具有一致性;共检测到17个叶绿体单倍型,其中,中国选育油橄榄品种仅有1个单倍型,其多数为地中海栽培油橄榄共享;野生油橄榄序列多态性最高,其次为地中海栽培油橄榄和伊朗种质,而9个中国选育品种的多样性为0。系统发育和单倍型网络关系结果一致,中国选育品种与多数地中海栽培油橄榄、伊朗种质聚为一支。【结论】中国选育油橄榄品种母系来源多样性极低,后期需从更全面的油橄榄种质谱系选育适应中国生境的油橄榄品种。
[Objective]The study aims to compare the genetic diversity and relationship between chloroplast genomes of olive varieties bred in China and those in the mediterranean olives,and to estimate the maternal diversity of olive varieties bred in China,in order to provide a basis for the development of Chinese olive accessions.[Methods]NOVOPlasty v.4.3.1 was used to assemble chloroplast genomes,which were based on the genome re-sequencing data of nine olive varieties bred in China and seven Iranian accessions,both of which were sequenced in this study,as well as fifty-two mediterranean olive accessions downloaded from the database.The sequence polymorphism was calculated.GPA,RAxML-NG,and Network 5.0,and other softwares were used to construct the chloroplast genome map,the relationship of phylogeny,and the network of haplotype,respectively.[Results]The chloroplast genomes of the tested olive accessions were consistent in terms of tetrad structure and number of genes.All chloroplast genomes identified seventeen haplotypes,among which the olive varieties bred in China had only one haplotype which was shared with the most mediterranean cultivated olives.The oleasters had the highest genetic di-versity,followed by the mediterranean cultivated olives and then Iranian accessions,while nine olive varie-ties bred held all genetic diversity of 0.The phylogenetic relationship was consistent with the network of haplotype,and one branch contained most of the mediterranean cultivated varieties,including the Iranian olive accessions and all olive varieties bred in China.[Conclusion]The nine olive varieties bred in China shows an extremely low diversity of maternal origin.It is necessary to select and breed the olive varieties adapted to the habitats in China from a more comprehensive lineage of olive accessions in the later stages.