文献类型：期刊文献

英文题名：Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo

作者：Yuan, Jin-Ling[1] Yue, Jin-Jun[1] Wu, Xiao-Li[1] Gu, Xiao-Ping[1]

第一作者：袁金玲

通信作者：Gu, XP[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang, Zhejiang, Peoples R China

年份：2013

卷号：8

期号：12

外文期刊名：PLOS ONE

收录：;Scopus(收录号：2-s2.0-84892621755);WOS:【SCI-EXPANDED(收录号:WOS:000328730300063)】；

基金：This research was supported by State Forestry Administration of the People's Republic of China, Project 948 (2011-4-49); Key Science and Technique Item of Zhejiang Province, Wood and Bamboo Breeding (2012C12908-3); Natural Science Foundation of Zhejiang Province (LQ12C16006). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Moso bamboo [Phyllostachys heterocycla var. pubescens (Mazel ex J. Houz.) Ohwi] is one of the most important forest crops in China and the rest of Asia. Although many sympodial bamboo tissue culture protocols have been established, there is no protocol available for plantlet regeneration as indicated by callus induction for monopodial bamboos, such as Moso bamboo. In the present report, embryogenic callus induction, embryoid development, and germination were established for Moso bamboo from zygotic seed embryos. Callus was initiated from zygotic embryos after 10-20 d culture on MS media supplemented with 4.0 mg/L 2, 4-D and 0.1 mg/L zeatin (ZT). About 50% of the explants produced calli, and nearly 15% of the calli were found to be embryogenic in nature. These embryogenic calli can be subcultured for proliferation in the Murashige and Skoog media (MS) supplemented with 0.5-2.0 mg/L 2, 4-D. These calli were found to have maintained their capacity for regeneration even after one year of subculture. The viable somatic embryoids regenerated in medium containing 5.0-7.0 mg/L ZT. Nearly 5% of the calli were found capable of regenerating into plantlets directly in MS medium containing 5.0-7.0 mg/L ZT. Root growth was more pronounced when the plantlets were transferred to medium containing 2.0 mg/L NAA. After 30 days of subculture, the plantlets were transferred to a greenhouse.