文献类型：期刊文献

英文题名：Analysis of the genetic diversity and molecular phylogeography of the endangered wild rose (Rosa rugosa) in China based on chloroplast genes

作者：Xu, Jianjun[1] Zang, Fengqi[2] Wu, Qichao[1] Wang, Yi[1] Wang, Baosheng[1] Huang, Ping[2] Zang, Dekui[1] Ma, Yan[1] Zheng, Yongqi[2]

第一作者：Xu, Jianjun

通信作者：Ma, Y[1];Zheng, YQ[2]

机构：[1]Shandong Agr Univ, Coll Forestry, Key Lab State Forestry Adm Silviculture Lower Yel, Tai An 271018, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Lab Forest Silviculture & Tree Cultivat, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2021

卷号：28

外文期刊名：GLOBAL ECOLOGY AND CONSERVATION

收录：;Scopus(收录号：2-s2.0-85110612142);WOS:【SCI-EXPANDED(收录号:WOS:000684512200006)】；

基金：This study was supported by the National Natural Science Foundation of China (31870688) and the Shandong Agricultural Seeds Engineering Project (2019LZGC01802). There was no additional external funding received for this study.

语种：英文

外文关键词：Chloroplast sequence; Endangered plant; Rosa rugosa; Genetic diversity; Molecular phylogeography

摘要：In this study, 3 chloroplast sequences (rnL-trnF, rpl20-rps12, and rbcl) were amplified and sequenced in 236 individuals from 12 wild rose (Rosa rugosa) populations in China to determine their genetic diversity, genetic differentiation and pedigree geographic structure in order to provide information necessary for formulating protection strategies. In total, 19 haplotypes were obtained. The gene flow (Nm) among the wild rose populations was 0.7, and the species exhibited low overall genetic diversity (Ht = 0.427 +/- 0.0863). The genetic differentiation coefficients of wild rose were Nst = 0.270 Gst = 0.197 (p < 0.01), indicating that the wild rose populations in China have obvious phylogeographic structure. Analysis of molecular variance (AMOVA) showed that genetic variation in wild rose occurs mainly within populations (77.27%) rather than between populations (22.73%). Furthermore, the wild rose populations exhibited a large degree of genetic differentiation (Fst = 0.2273). The results of a Mantel test showed that geographical isolation was not the main factor causing the genetic differentiation among the wild rose populations. A mismatch analysis curve and neutrality test indicated that the wild rose populations recently expanded. There may be multiple refuges for wild rose, such as the Shandong Yantai (DYZ) population, the Weihai (CSZ) population in the south, and the Hunchun (JXZ) population in the north. Orogeny, the formation of the Bohai Sea, and human activities are important drivers of the fragmented distribution of wild rose in China.