文献类型：期刊文献

英文题名：Salty treatment increased bioactive compounds accumulation during agarwood development in Aquilaria sinensis trees

作者：Yan, Tingting[1] Zhang, Ningnan[2] Hong, Zhou[2] Chen, Yuan[1] Li, Gaiyun[1]

第一作者：晏婷婷

通信作者：Li, GY[1]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China

年份：2024

卷号：175

外文期刊名：FITOTERAPIA

收录：;Scopus(收录号：2-s2.0-85188535349);WOS:【SCI-EXPANDED(收录号:WOS:001222435300001)】；

基金：This work was supported by the special funds for basic scientific research business expenses of the Key Research and Development Project of Hainan Province (ZDYF2022SHFZ044) and basic scientific research business expenses of central public welfare scientific research institutes from Chinese Academy of Forestry (CAFYBB2021QD003) .

语种：英文

外文关键词：Agarwood inducing method; Bioactive compounds; 2D-TLC-EDA; PECs

摘要：To compare the bioactive compounds in agarwood induced by different methods in Aquilaria sinensis(Lour.) Gilg trees, a two dimensional thin layer chromatograph(2D-TLC) combined with effect directive analysis(EDA) was developed. Three antioxidants were found by 2D-TLC-DPPH and further identified as 2-(2-phenylethyl) chromones(PECs) with LC-MS/MS. The 3 antioxidants decreased along agarwood formation and their compositions in drilling induced agarwood differed with those in microbe culture induced agarwood. Further study showed NaCl treatment promoted antioxidants accumulation in agarwood induced by drilling or hot drilling. Hot drilling combined with salty stimulation was most efficient in some chemicals accumulation, which were identified as PECs with antioxidant, tyrosinase or beta-glucosidase inhibiting activities by 2D-TLC-EDA-LC-MS/MS. This study provided a 2D-TLC-EDA-LC-MS/MS method for bioactive compounds screen and qualification of agarwood. Based on this method, non-conventional methods were found to accelerate the accumulation of some bioactive PECs in A. sinensis trees.