文献类型：期刊文献

英文题名：SpbZIP60 confers cadmium tolerance by strengthening the root cell wall compartmentalization in Sedum plumbizincicola

作者：Yu, Zhuchou Lua Miao[1,2] Han, Xiaojiao[1] Qiao, Guirong[1] Xu, Jing[1] Wu, Longhua[3] Qiu, Wenmin[1] Zhuo, Renying[1]

第一作者：Yu, Zhuchou Lua Miao

通信作者：Zhuo, RY[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[2]Fujian Acad Forestry Sci, Fuzhou 350012, Fujian, Peoples R China;[3]Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China

年份：2024

卷号：480

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20243917091760);Scopus(收录号：2-s2.0-85204441800);WOS:【SCI-EXPANDED(收录号:WOS:001322713600001)】；

基金：This work was supported by National Key Research and Develop-ment Program of China (No. 2021YFD2200201) to RZ.

语种：英文

外文关键词：Sedum plumbizincicola; bZIP60; Cd tolerance; Transcriptional regulation; Cell wall; Lignin deposition

摘要：Cadmium (Cd) is a prominent heavy metal pollutant that inhibits plant growth and poses risks to human health. Sedum plumbizincicola, as a Cd/Zn/Pb hyperaccumulator species, exhibits robust resistance to heavy metals and effective enrichment capacities. In our previous study, overexpressing SpbZIP60 in Arabidopsis enhanced Cd tolerance; however, the underlying the molecular mechanism remains to be elucidated. Here, we identified SpbZIP60 as a representative Cd stress response factor with nuclear localization and transcriptional activation activity. SpbZIP60 underwent conservative splicing in response to endoplasmic reticulum (ER) stress, while its response to Cd stress is independent of the ER stress-mediated unfolded protein response pathway. Overexpression of SpbZIP60 in S. alfredii increased the Cd tolerance and antioxidant activity. Furthermore, SpbZIP60 increased the content of cell wall components and thickened cell wall under Cd stress. Transcriptome analysis indicated a significant enrichment of differentially expressed genes within the phenylpropanoid metabolism pathway. Besides, the binding of SpbZIP60 to the promoter region of SpBglu resulted in the activation of gene expression, thereby enhancing the process of lignin deposition. Collectively, our results elucidated a molecular regulatory model in which SpbZIP60 increased the thickness of the root cell wall to impede Cd entry into the cell, consequently improving Cd tolerance.