文献类型：期刊文献

英文题名：Comparison of Cold Hardiness Evaluation of Woody Species by ELLT and TTCLT

作者：Li, Hui-qing[1] Li, Qing-he[1] Xing, Lei[1] Sun, Gao-jie[1] Zhao, Xiu-lian[1]

第一作者：李慧卿

通信作者：Li, HQ[1];Li, QH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2020

卷号：55

期号：8

起止页码：1228-1232

外文期刊名：HORTSCIENCE

收录：;Scopus(收录号：2-s2.0-85091586690);WOS:【SCI-EXPANDED(收录号:WOS:000557226100005)】；

基金：This work was supported by Basic Scientific Research Fund of CAF (CAFYBB2017MA021), National Science and Technology Basic Resources Survey (2017FY10020404), and Central Public-interest Scientific Institution Basic Research Fund (CAFYBB2017SY003).

语种：英文

外文关键词：cambium; electrolyte leakage; phloem; pith; TTC; xylem

摘要：Cold hardiness evaluation is important for screening woody species in cold areas. We compared cold hardiness by estimating the 50% lethal temperature (LT50) using electrolyte leakage test (ELLT50) and triphenyltetrazolium chloride test (TTCLT50) for 26 woody species in the Bashang region of China. One-year-old shoots were collected in January and exposed to five subfreezing temperatures in a programmable temperature and humidity chamber. LT50 was estimated by fitting relative electrolyte leakage and percentage of dead tissue against test temperature. For all tested species, triphenyltetrazolium chloride (TTC) staining of the pith was weak and the cambium TTCLT50 was lower than the extreme minimum temperature (- 37 degrees C) recorded in the region. The cambium TTCLT50 and the SD were lower than that for the phloem and xylem. The phloem TTCLT50 was lower than the xylem TTCLT50, and the two sos were similar. The ELLT50 showed no significant correlation with any TTCLT50. For most species, the ELLT50 was higher than the cambium and phloem TTCLT50 and was not significant different with the xylem TTCLT50. The ELLT50 showed higher SD than any tissue TTCLT50. Based on results obtained in this study, when choosing cold hardiness of single stem tissue as an indicator for screening woody species, the xylem should be considered first, followed by the phloem; the cambium and pith were unsuitable. The cold hardiness estimated by ELLT50 was more suitable as indicator for screening woody species than that of stem tissue in winter estimated by TTCLT50.