文献类型：期刊文献

中文题名：不同接种和处理方式对巨桉幼苗青枯病发生的影响

英文题名：Effect of Ralstonia solanacearum Infecting Eucalyptus grandis by Different Treatments and Inoculation Methods

作者：范春节[1] 姚海荣[1,2,3] 曾炳山[1] 王胜坤[1] 郭光生[1] 覃伟权[3]

第一作者：范春节

机构：[1]中国林业科学研究院热带林业研究所;[2]海南大学环境与植物保护学院;[3]中国热带农业科学院椰子研究所

年份：2016

卷号：37

期号：8

起止页码：1547-1552

中文期刊名：热带作物学报

外文期刊名：Chinese Journal of Tropical Crops

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家科技计划项目(No.2013AA102705);中央级公益性科研院所基本科研业务费专项资金项目(No.RITFYWZX201304)

语种：中文

中文关键词：巨桉;桉树青枯病;青枯雷尔氏菌

外文关键词：Eucalyptus grandis; Eucalyptus bacterial wilt; Ralstonia solanacearum

分类号：S763.7

摘要：桉树青枯病的发生由于影响因素较多导致其人工接种重复性差,因此需要获得稳定的青枯病感染,同时在此基础上可以进行桉树苗期青枯病的防治研究。在本研究中采用水培后伤新根和袋苗未经水培直接伤根后转移到青枯菌液中浸泡6、12、18、24 h后移植,调查桉树幼苗青枯病发病率和生长状况,同时通过在接种前喷施不同浓度的BR、Me JA、SA 3种激素,诱导桉树抵抗青枯菌侵染,调查处理后苗木死亡率。结果表明,未经过水培直接伤根移植的苗木更容易感染青枯菌,经过24 h菌液浸泡处理后的幼苗的死亡率,达到86.67%,同时发现SA对缓解桉树青枯病的发病有明显的作用,且在喷施浓度为0.001 mmol时使用,青枯菌侵染的植株仍有75.0%的存活率。本研究获得了桉树幼苗稳定感染青枯病的一种方式,为室内研究桉树幼苗感染青枯病的机制奠定基础,同时获得了降低桉树青枯病发生的处理方式,为桉树苗期青枯病的防治提供一种方案。
To study the mechanism of denfending Eucalyptus bacterial wilt, obtaining an efficient inoculation method for Ralstonia solanacearum on Eucalyptus was necessary owing to its scarce and low replicability. On the basis of this, different concentrations of BR, MeJA and SA treatments were used to improve Eucalyptus resistance for R. solanacearum. In this study, immersion of wounded roots by water culture and soil culture in bacterial cell suspension 6 hours, 12 hours, 18 hours and 24 hours were tested. The plants wounded roots by soil culture in bacterial cell suspension 24 hours were proved to be the most efficient for inoculating Eucalyptus with R. solanacearum and the rate of death reached the highest for 86.67%. Meanwhile, it was found that SA treatments increased defence to R. solanacearum individually. Moreover, SA significantly increased the rate of survive by full coverage foliage spray before plants inoculated with R. solanacearum, which 0.001 mmol SA treatment gained the major improvement for 75.0% survival. This study developed an effective protocol for inoculation of R. solartacearum, which based for further investigated the mechanism of R. solanacearum infect Eucalyptus and supplied a method for affect against Eucalyptus bacterial wilt.