文献类型：期刊文献

英文题名：Classification of Calcium-Dependent Protein Kinases and Their Transcriptional Response to Abiotic Stresses in Halophyte Nitraria sibirica

作者：Lu, Lu[1,2] Chen, Ting[1,2] Yang, Tiangui[1,2] Han, Chunxia[3] Zhang, Jingbo[3] Chen, Jinhui[1,2] Cheng, Tielong[4]

第一作者：Lu, Lu

通信作者：Cheng, TL[1]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, State Key Lab Tree Genet & Breeding, Nanjing 210037, Peoples R China;[2]Nanjing Forestry Univ, Key Lab Forest Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou 015200, Peoples R China;[4]Nanjing Forestry Univ, Coll Ecol & Environm, Nanjing 210037, Peoples R China

年份：2025

卷号：14

期号：19

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-105018944836);WOS:【SCI-EXPANDED(收录号:WOS:001593538700001)】；

基金：This research was supported by the Youth Foundation of Nature Science Foundation of Jiangsu Province (BK20230390) and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：calcium-dependent protein kinase; halophyte; Nitraria sibirica; abiotic stress; salinity

摘要：Calcium-dependent protein kinases (CDPKs) are key Ca2+ sensors in plants, mediating responses to abiotic stresses via phosphorylation signaling. In the halophyte Nitraria sibirica, which thrives in saline soils, we identified 19 CDPK genes (NsCDPKs) and classified them into four canonical angiosperm clades, highlighting conserved functional modules. Promoter analysis revealed diverse cis-acting elements responsive to light, hormones (ABA, MeJA, auxin, GA, SA), and abiotic stresses (drought, cold, wounding), along with numerous MYB binding sites, suggesting complex transcriptional regulation. Transcriptome profiling under salt stress (100 and 400 mM NaCl) showed induction of most NsCDPKs, with several genes significantly upregulated in roots and stems, indicating coordinated whole-plant activation. These salt-responsive NsCDPKs were also upregulated by cold but repressed under PEG-simulated drought, indicating stress-specific regulatory patterns. Fifteen MYB transcription factors, differentially expressed under salt stress, were predicted to interact with NsCDPK promoters, implicating them as upstream regulators. This study identified a potential salt- and cold-responsive CDPK regulatory module and a MYB-mediated transcriptional hierarchy in N. sibirica, providing insights into the molecular mechanisms of salinity adaptation and highlighting candidate genes that could be explored for improving salt tolerance in crop species.