文献类型：期刊文献

中文题名：基于DNS比色法的土壤蔗糖酶检测条件选择

英文题名：Soil Sucrase: Detection Conditions Based on DNS Colorimetric

作者：李朝英[1] 郑路[1]

第一作者：李朝英

机构：[1]中国林业科学研究院热带林业实验中心/国家林业局友谊关森林生态系统定位研究站

年份：2016

卷号：32

期号：27

起止页码：171-176

中文期刊名：中国农学通报

外文期刊名：Chinese Agricultural Science Bulletin

收录：CSTPCD

基金：广西自然科学基金"马尾松人工林采伐剩余物分解及提升地力研究"(2013GXNSFAA019106)

语种：中文

中文关键词：DNS比色法;土壤;蔗糖酶

外文关键词：DNS colorimetry; soil; sucrase

分类号：S151.94

摘要：为了准确测定土壤蔗糖酶含量,分析了不同波长、显色剂加入量、甲苯加入量、加热时间、显色稳定时间等条件对3,5-二硝基水杨酸（3,5-dinitrosalicylic acid,DNS）比色法测定土壤蔗糖酶含量的影响。结果表明：当波长为510 nm,显色剂用量为1.5 m L,甲苯加入量0.1 m L,水浴时间5 min,待测液存放时间小于4 h,显色稳定时间在0.5~2 h之间时,标准曲线在0.25~2 mg范围内线性良好（R2=0.9991）,土壤蔗糖酶含量的变异系数（CV）小于9%,回收率在96%~109%范围内,检测结果的精密度和准确性较高。上述检测方法安全、经济、高效,适用于实验室批量土壤的蔗糖酶含量测定。
The aim was to detect the content of soil sucrase precisely. The effect of different wavelengths, theamount of chromogenic agent and toluene, heating time and the stable time of color on the content of soilsucrase detected by 3,5-2 dinitrosalicylic acid（DNS） method was analyzed. The results showed that when thewavelength was 510 nm, the amount of reagent was 1.5 m L, the amount of toluene was 0.1 m L, the time of waterbath was 5 min, storage time of the solution to be determined was less than 4 h, the stable time of color wasbetween 0.5- 2 h, the standard curve in the range of 0.25- 2 mg had good linear relation（R2=0.9991）, thecoefficient of variation（CV） was less than 9%, the recoveries were in the range of 96%- 109%, and theprecision and accuracy of the test results were relatively high. The above method was safe, economical andefficient, and suitable for batch determination of soil sucrase.