文献类型：期刊文献

英文题名：Comprehensive evaluation of fuel properties and complex regulation of intracellular transporters for high oil production in developing seeds of Prunus sibirica for woody biodiesel

作者：Wang, Jia[1] Lin, Weijun[2] Yin, Zhongdong[1] Wang, Libing[3] Dong, ShuBin[1] An, Jiyong[1] Lin, Zixin[1] Yu, Haiyan[3] Shi, Lingling[1] Lin, Shanzhi[1] Chen, Shaoliang[1]

第一作者：Wang, Jia

通信作者：Lin, SZ[1];Chen, SL[1]

机构：[1]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding, Coll Biol Sci & Biotechnol,Sch Soil & Water Conse, Beijing 100083, Peoples R China;[2]Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2019

卷号：12

外文期刊名：BIOTECHNOLOGY FOR BIOFUELS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000454948200007)】；

基金：This research was supported by the National Natural Sciences Foundation of China (No. 31570653) and Beijing Advanced Innovation Center for Tree Breeding by Molecular Design.

语种：英文

外文关键词：Prunus sibirica seeds; Biodiesel fuel properties; Oil synthesis; Intracellular transporter; Differential transcript profiles; Transporter-mediated mechanism

摘要：BackgroundBased on our previous studies of 17 Prunus sibirica germplasms, one plus tree with high quality and quantity of seed oils has emerged as novel potential source of biodiesel. To better develop P. sibirica seed oils as woody biodiesel, a concurrent exploration of oil content, FA composition, biodiesel yield and fuel properties as well as prediction model construction for fuel properties was conducted on developing seeds to determine the optimal seed harvest time for producing high-quality biodiesel. Oil synthesis required supply of carbon source, energy and FA, but their transport mechanisms still remains enigmatic. Our recent 454 sequencing of P. sibirica could provide long-read sequences to identify membrane transporters for a better understanding of regulatory mechanism for high oil production in developing seeds.ResultsTo better develop the seed oils of P. sibirica as woody biodiesel, we firstly focused on a temporal and comparative evaluation of growth tendency, oil content, FA composition, biodiesel yield and fuel properties as well as model construction for biodiesel property prediction in different developing seeds from P. sibirica plus tree (accession AS-80), revealing that the oils from developing seeds harvested after 60days after flowering (DAF) could be as novel potential feedstock for producing biodiesel with ideal fuel property. To gain new insight into membrane transport mechanism for high oil yield in developing seeds of P. sibirica, we presented a global analysis of transporter based on our recent 454 sequencing data of P. sibirica. We annotated a total of 116 genes for membrane-localized transporters at different organelles (plastid, endoplasmatic reticulum, tonoplast, mitochondria and peroxisome), of which some specific transporters were identified to be involved in carbon allocation, metabolite transport and energy supply for oil synthesis by both RT-PCR and qRT-PCR. Importantly, the transporter-mediated model was well established for high oil synthesis in developing P. sibirica seeds. Our findings could help to reveal molecular mechanism of increased oil production and may also present strategies for engineering oil accumulation in oilseed plants.ConclusionsThis study presents a temporal and comparative evaluation of developing P. sibirica seed oils as a potential feedstock for producing high-quality biodiesel and a global identification for membrane transporters was to gain better insights into regulatory mechanism of high oil production in developing seeds of P. sibirica. Our findings may present strategies for developing woody biodiesel resources and engineering oil accumulation.