文献类型：期刊文献

英文题名：Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

作者：Du, Changjian[1] Chen, Wei[1] Wu, Yanyan[1] Wang, Guangpeng[2] Zhao, Jiabing[3] Sun, Jiacheng[1] Ji, Jing[1] Yan, Donghui[4] Jiang, Zeping[4] Shi, Shengqing[1]

第一作者：Du, Changjian

通信作者：Shi, SQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, 1958 Box, Beijing 100091, Peoples R China;[2]Hebei Acad Agr & Forestry Sci, Inst Pomol, Changli 066600, Peoples R China;[3]Hebei Agr Univ, Coll Forestry, Baoding 071001, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Key Lab Forest Ecol & Environm Natl Forestry & Gr, 1958 Box, Beijing 100091, Peoples R China

年份：2020

卷号：9

期号：4

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-85084216782);WOS:【SCI-EXPANDED(收录号:WOS:000539578900043)】；

基金：This work was supported by grants from the Project of Science and Technology Development Center of State Forestry and grassland administration (KJZXSA2019045); the National Natural Science Foundation of China (31971627); the Project of Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration (ZDRIF201712); the Science & Technology Research and Development Program of Hebei Province (16226312D).

语种：英文

外文关键词：chestnut; GABA; seed germination; carbon metabolism; nitrogen metabolism

摘要：Recalcitrant chestnut seeds are rich in gamma-aminobutyric acid (GABA), which negatively regulates adventitious root development by altering carbon/nitrogen metabolism. However, little is known regarding the role of this metabolite in chestnut seeds. In this study, we investigated the effects of GABA changes on the germination of chestnut seeds treated with exogenous GABA and vigabatrin (VGB, which inhibits GABA degradation). Both treatments significantly inhibited seed germination and primary root growth and resulted in the considerable accumulation of H2O2, but the endogenous GABA content decreased before germination at 48 h. Soluble sugar levels increased before germination, but subsequently decreased, whereas starch contents were relatively unchanged. Changes to organic acids were observed at 120 h after sowing, including a decrease and increase in citrate and malate levels, respectively. Similarly, soluble protein contents increased at 120 h, but the abundance of most free amino acids decreased at 48 h. Moreover, the total amino acid levels increased only in response to VGB at 0 h. Accordingly, GABA and VGB altered the balance of carbon and nitrogen metabolism, thereby inhibiting chestnut seed germination. These results suggested that changes to GABA levels in chestnut seeds might prevent seed germination. The study data may also help clarify the dormancy and storage of chestnut seeds, as well as other recalcitrant seeds.