文献类型：期刊文献

英文题名：Analyses of high spatial resolution datasets identify genes associated with multi-layered secondary cell wall thickening in Pinus bungeana

作者：Guo, Yu[1,2] Jiao, Lichao[1,2] Wang, Jie[1,2] Ma, Lingyu[1,2] Lu, Yang[1,2] Zhang, Yonggang[1,2] Guo, Juan[1,2] Yin, Yafang[1,2]

第一作者：郭雨;Guo, Yu

通信作者：Guo, J[1];Yin, YF[1];Guo, J[2];Yin, YF[2]

机构：[1]Chinese Acad Forestry, Wood Anat & Utilizat Dept, Res Inst Wood Ind, Beijing 100091, Peoples R China;[2]Natl Forestry & Grassland Adm, Wood Specimen Resource Ctr WOODPEDIA, Beijing 100091, Peoples R China

年份：2024

卷号：133

期号：7

起止页码：953-968

外文期刊名：ANNALS OF BOTANY

收录：;Scopus(收录号：2-s2.0-85193103881);WOS:【SCI-EXPANDED(收录号:WOS:001187811100001)】；

基金：This work was supported by National Natural Science Foundation of China (grant number 32071691) and the Department of Science and Technology, National Forestry and Grassland Administration (2020132601).

语种：英文

外文关键词：Pinus bungeana; secondary cell wall thickening; wood anatomy; wood formation; high spatial resolution; RNA sequencing; regulation network; transcription factor; cambium cell; mature wood

摘要：Background and Aims Secondary cell wall (SCW) thickening is a major cellular developmental stage determining wood structure and properties. Although the molecular regulation of cell wall deposition during tracheary element differentiation has been well established in primary growth systems, less is known about the gene regulatory processes involved in the multi-layered SCW thickening of mature trees.Methods Using third-generation [long-read single-molecule real-time (SMRT)] and second-generation [short-read sequencing by synthesis (SBS)] sequencing methods, we established a Pinus bungeana transcriptome resource with comprehensive functional and structural annotation for the first time. Using these approaches, we generated high spatial resolution datasets for the vascular cambium, xylem expansion regions, early SCW thickening, late SCW thickening and mature xylem tissues of 71-year-old Pinus bungeana trees.Key Results A total of 79 390 non-redundant transcripts, 31 808 long non-coding RNAs and 5147 transcription factors were annotated and quantified in different xylem tissues at all growth and differentiation stages. Furthermore, using this high spatial resolution dataset, we established a comprehensive transcriptomic profile and found that members of the NAC, WRKY, SUS, CESA and LAC gene families are major players in early SCW formation in tracheids, whereas members of the MYB and LBD transcription factor families are highly expressed during late SCW thickening.Conclusions Our results provide new molecular insights into the regulation of multi-layered SCW thickening in conifers. The high spatial resolution datasets provided can serve as important gene resources for improving softwoods.