文献类型：期刊文献

英文题名：Bacillus thuringiensis Cry3Aa fused to a cellulase-binding peptide shows increased toxicity against the longhorned beetle

作者：Guo, Chang-Hua[2,3] Zhao, Shu-Tang[1,3] Ma, Yuan[3] Hu, Jian-Jun[1,3] Han, Xiao-Jiao[2,3] Chen, Jun[1,3] Lu, Meng-Zhu[1,3]

第一作者：Guo, Chang-Hua

通信作者：Lu, MZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2012

卷号：93

期号：3

起止页码：1249-1256

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000300282500033)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 30872049) and Transgenic Breeding Projects (No. 2009ZX08009-095B), Ministry of Agriculture, China.

语种：英文

外文关键词：Binding peptide; Cry3Aa; Cx-cellulase; Enhancement; Longhorned beetle

摘要：Cry3 class toxins are used extensively for biological control of coleopteran larvae. We previously identified a peptide (PCx) from a phage display library that specifically binds Cx-cellulase from the midgut of Anoplophora glabripennis Motschulsky (Asian longhorn beetle) larvae. Here, we added a DNA fragment that encodes the peptide onto either end of the cry3Aa gene and tested the expressed PCx-Cry3Aa and Cry3Aa-PCx proteins for insecticidal activity in the longhorned beetle. An insect bioassay revealed that, compared with native Cry3Aa, the two modified Cry3Aa proteins had significantly higher lethality, with PCx-Cry3Aa exhibiting a mortality rate almost three times that of Cry3Aa. We also proposed that the increased lethality in larvae fed with PCx-Cry3Aa or Cry3Aa-PCx would be attributable to the binding of the toxin with Cx-cellulase, thereby increasing toxin retention in the midgut. The significantly enhanced insecticidal activity of Cry3Aa fused with the Cx-cellulase binding peptide provides a new strategy for increasing toxin efficacy against the longhorned beetle. These uniquely modified Cry3Aa proteins have potential use for pest control.