文献类型：期刊文献

中文题名：地被银桦试管苗增殖培养研究

英文题名：Proliferation of Grevillea ‘Billy Bonkers' via Tissue Culture

作者：李湘阳[1] 曾炳山[1] 裘珍飞[1]

第一作者：李湘阳

机构：[1]中国林科院热带林业研究所

年份：2016

卷号：36

期号：10

起止页码：28-32

中文期刊名：热带农业科学

外文期刊名：Chinese Journal of Tropical Agriculture

收录：CSTPCD

基金：948引进项目"地被银桦新品种及繁育技术引进"(No.2012-4-63)

语种：中文

中文关键词：地被银桦;组织培养;增殖

外文关键词：Grevillea ＇Billy Bonkers＇; tissue culture; proliferation

分类号：Q813.1

摘要：地被银桦是杂交品种,无性繁殖对保持地被银桦的品种特性至关重要。本研究以组织培养为繁殖方法,从激素的浓度、种类、大量元素及糖等方面对地被银桦的增殖培养进行了系统的研究。结果表明,6-BA的浓度对增殖苗的高度有显著影响,6-BA和IBA对试管苗的玻璃化比例均有显著影响,NH_4NO_3和KH_2PO_4的浓度也显著影响增殖苗的高度。最适增殖培养基配方为:改良MS培养基(NH_4NO_3412.5 mg/L,KH_2PO_485 mg/L)+6-BA 0.3 mg/L+IBA 0.1 mg/L+糖35 g/L。
Grevillea ＇Billy Bonkers＇ is a hybrid Grevillea. Vegetative propagation plays an important role in keeping varietal characteristics of ＇Billy Bonkers＇. ＇Billy Bonkers＇ was mass proliferated via tissue culture, and the hormones and their concentrations, macro-elements and sugar were modified to develop an optimal MS medium for tissue culture of ＇Billy Bonkers＇. The results indicated that the concentrations of 6BA, NH_4NO_3 and KH_2PO_4had significant effect on plantlet height growth of ＇Billy Bonkers＇. The 6BA and IBA had significant effect on plantlet vitrification ratio. The optimal medium for tissue culture is（NH_4NO_3412.5 mg/L, KH_2PO_485 mg/L） ＋ 6BA 0.3 mg/L ＋ IBA 0.1 mg/L ＋ sugar 35 g/L.