文献类型：期刊文献

中文题名：金银花黑斑病病原菌番茄匍柄霉的分离与鉴定

英文题名：Isolation and Identification of Pathogenic Fungus Stemphylium lycopersici Causing the Black Spot Disease on Honeysuckle(Lonicera japonica Thunb.)

作者：刘端冲[1] 杨金库[2] 林若竹[1] 姚艳霞[1] 淮稳霞[1] 赵文霞[1]

第一作者：刘端冲

机构：[1]国家林业和草原局森林保护学重点实验室?中国林业科学研究院森林生态环境与自然保护研究所,北京100091;[2]廊坊市农林科学院,河北?廊坊065000

年份：2023

卷号：36

期号：6

起止页码：30-39

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：中央级公益性科研院所基本科研业务费专项“塞罕坝重大林业灾害防控技术研究”(项目编号CAFYBB2018ZB005)。

语种：中文

中文关键词：金银花;黑斑病;番茄匍柄霉

外文关键词：Lonicera japonica Thunb.;black spot;Stemphylium lycopersici

分类号：S763.15

摘要：[目的]分离并鉴定引起金银花黑斑病的病原菌,为该病害的有效防控提供理论依据。[方法]采用常规组织分离法对发病金银花叶片进行分离,结合形态学、多基因序列分析(ITS、gpd和EF-1α序列)和致病性测定进行病原物种鉴定。[结果]在形态学上,分离获得的菌株YP4在PDA培养基上的菌落呈灰白或黄白色棉毛状,与番茄匍柄霉的菌落特征相符;在基于ITS、gpd和EF-1α序列联合构建的3种系统发育树中,供试菌株YP4均以较高支持率与番茄匍柄霉(Stemphylium lycopersici)聚在同一分支上;致病性接种实验结果表明,菌株YP4接种至金银花叶片上和番茄叶片上均可产生病斑,并可再分离获得其纯菌株;因此,结合形态学、分子系统发育学以及致病性测定,该病原真菌被鉴定为番茄匍柄霉。[结论]本研究明确了引起河北省金银花黑斑病的病原菌为番茄匍柄霉,这是该病菌在我国危害金银花的首次报道。
[Objective]To clarify the pathogen causing black spot disease on Lonicera japonica,and to provide a theoretical basis for effective prevention and control of this disease.[Method]The strains of the pathogen were isolated by conventional tissue plate method,and were identified by the morphological characters and phylogenetic analysis of ITS,gpd and EF-1αsequences.Its pathogenicity was tested through in vitro inoculating healthy leaves of L.japonica and Solanum lycopersicum.[Result]The isolated fungal strain YP4 morphologically formed greyish to yellowish white,cottony colonies on Potato Dextrose Agar,which were consistent with published descriptions of Stemphylium lycopersici.In the three phylogen-etic trees based on ITS,gpd and EF-1αsequences,the isolate YP4 fell into the S.lycopersici clade with strong support.Based on the pathogenicity measurement of Koch's rule,the strain YP4 was pathogenic to leaves of L.japonica and S.lycopersicum.[Conclusion]Combined with the morphological characteristics,the pathogenicity determination and phylogenetic analysis of ITS,gpd and EF-1αsequences,the patho-gen causing black spot disease on honeysuckle is identified as S.lycopersici.To our knowledge,this is the first report of S.lycopersici causing black spot of L.japonica in China.