文献类型：期刊文献

英文题名：Transcriptome Analysis Provides Insights into Korean Pine Tree Aging and Response to Shading

作者：Ye, Zha-Long[1] Liu, Jin-Yi[2] Feng, Jian[3] Li, Wan-Feng[1]

第一作者：Ye, Zha-Long

通信作者：Li, WF[1];Feng, J[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]State Owned Dabiangou Forest Farm Qingyuan Man Aut, Fushun 113000, Peoples R China;[3]Liaoning Acad Forest Sci, Shenyang 110000, Peoples R China

年份：2024

卷号：15

期号：2

外文期刊名：FORESTS

收录：;EI(收录号：20240915640746);Scopus(收录号：2-s2.0-85185848346);WOS:【SCI-EXPANDED(收录号:WOS:001171784100001)】；

基金：The authors would like to thank Xiang-Yi Li (Research Institute of Forestry, Chinese Academy of Forestry) and the staff at Dabiangou Forest Farm for their help with sample collection. They also wish to thank I.C. Bruce (Peking University) for conducting a critical reading of the manuscript.

语种：英文

外文关键词：adaptation; age; conifer; shading; silviculture; transcriptome

摘要：Age controls a tree's responses to environmental cues and shading influences tree growth and physiology. These are basic principles of "Afforestation under canopy", an approach that is widely used in the regeneration of Korean pine forests. Studying the underlying mechanism helps us to understand tree adaptation and utilize it in forest management. In this study, we investigated the transcriptomic changes in the uppermost main stems of the Korean pine tree (Pinus koraiensis, Sieb. et Zucc.) at different ages (5, 7, 10, 14, and 17 years) and in different growth conditions (open-grown and shade-grown trees) using RNA-Seq. In total, 434,005,837 reads were produced and assembled into 111,786 unigenes. After pairwise comparisons, 568 differentially expressed unigenes (DEUs) were identified. The greatest number of DEUs was identified in the comparison between 5-year-old open-grown trees and 17-year-old shade-grown trees, while no DEUs were identified in 15 pairwise comparisons. Among these 568 DEUs, 45 were assigned to gene ontology (GO) terms associated with response to environmental changes, including "response to stress" (26) and "response to light and temperature" (19); 12 were assigned to GO terms associated with sexual reproduction, such as "sexual reproduction", "specification of floral organ identity", "pollen tube guidance", and "fruit ripening"; 15 were heat shock protein genes and showed decreased expression patterns with age; and one, annotated as Pinus tabuliformis DEFICIENS-AGAMOUS-LIKE 1, showed an increased expression pattern with age, independent of the reproductive state or growth conditions of Korean pine trees. Altogether, these findings not only demonstrate the molecular aspects of the developmental and physiological effects of age and shading on Korean pine trees, but also improve our understanding of the basic principles of "Afforestation under canopy".