详细信息
文献类型:期刊文献
中文题名:红花石蒜遗传多样性的ISSR分析
英文题名:Analysis of Genetic Diversity in Lycoris radiata Using ISSR Marker
作者:左慧[1,2] 杨志玲[1] 杨旭[1] 谭梓峰[1] 于华会[1]
第一作者:左慧
机构:[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400;[2]中南林业科技大学,湖南长沙410004
年份:2008
卷号:21
期号:6
起止页码:768-772
中文期刊名:林业科学研究
外文期刊名:Forest Research
收录:CSTPCD;;Scopus;北大核心:【北大核心2004】;CSCD:【CSCD2011_2012】;
基金:国家科技部公益性林业专项项目“南方林源多用途药用植物种质保护和选育技术”(200704022);浙江省林业厅财政补助专项项目“林药(石蒜)生态经济型培育模式关键技术研究与示范”(07A03);浙江省重点农业项目“药用红花石蒜优质资源选育、野生驯化培育及离体培养再生体系的建立”(2004C22041);中国林科院亚热带林木培育实验室项目(特色药用植物专类园建设)部分内容
语种:中文
中文关键词:红花石蒜;ISSR;遗传多样性;遗传结构;遗传分化
外文关键词:Lycoris radiata ; ISSR; genetic diversity ; genetic structure ; genetic differentiation
分类号:S682.29
摘要:利用ISSR分子标记技术对14个居群的红花石蒜进行遗传多样性研究,结果表明:POPGEN32分析显示红花石蒜物种的遗传多样性很高,多态位点百分率为92.31%,Shannon指数(H)为0.459 7,Ne i指数(I)为0.302 5;居群水平的遗传多样性较低,多态位点百分率平均为49.65%,Shannon指数(H)平均为0.262 0,Ne i指数(I)平均为0.176 3;居群间的遗传分化系数(Gst)为0.503 5,基因流(Nm)为0.698 3。AMOVA分子变异分析显示:居群间遗传分化程度高,46.12%的变异发生在居群内,53.88%的变异发生于居群间。生境的片段化使居群间的基因流受阻,可能是居群间高遗传分化和居群水平低遗传多样性的主要原因。
The genetic diversity of 14 Lycoris radiata populations was analyzed by ISSR markers. The results of POPGEN32 analysis showed that at species level, the percentage of polymorphics was 92.31%, Shannon' s index (H) was 0. 459 7, and Nei' s gene diversity (I) was 0. 302 5, indicating a high level of genetic diversity. But at population level, they were 49.65% ,0. 262 0 and 0. 176 3, respectively, suggesting a low level of genetic diversity. And the gene differentiation coefficient (Gst) and gene flow ( Nm ) among the populations were 0. 503 5 and 0.698 3, respectively. The analysis of molecular variance (AMOVA) demonstrated that there was a relatively high level genetic variation(46.12% ) among the populations, and there was relatively low genetic variation (53.88%) within the populations. The high genetic differentiation among populations and the low genetic diversity within populations could be attributed to the habitat fragmentation and the limited gene flow among populations.
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