文献类型：期刊文献

英文题名：Polyploidy induction of clone of Eucalyptus grandis with colchicine

作者：Han, C.[1] Xu, J. M.[1] Du, Z. H.[1] Li, G. Y.[1] Zeng, B. S.[1] Wu, S. J.[1] Wang, W.[1]

第一作者：韩超

通信作者：Xu, JM[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2011

卷号：10

期号：66

起止页码：14711-14717

外文期刊名：AFRICAN JOURNAL OF BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000298549800004)】；

基金：The study was supported by grant from the project of National Key Technology R&D Program for the 12th five-year plan (China): "Breeding of High Yield and High Resistance Fast-growing Wood Species of Eucalyptus".

语种：英文

外文关键词：Colchicine; polyploidy; eucalyptus; osmotic agent

摘要：The study was conducted to find the dependency of polyploid inductivity rate of axillary buds of Eucalyptus grandis clone Eg5 with colchicine on treatment conditions by applying Plackkett-Burman design and uniform design. According to regression analysis, the functional relation equation between polyploid inductivity rate and independent variables including colchicines concentration and soaking time was found with Adj R(2) as 0.9366 which is ideal. The maximum inductivity rate can reach 51% when the soaking time is 26.2 h and the colchicines concentration is 0.21%. The study result shows that inductivity rises at first and drops later with continuous increases of colchicine concentration and soaking time. The inductivity drops, because of the mass mortality of treated axillary buds. The difference of tree height between tetraploid plants and untreated plants was significant while the difference of ground diameter between them did not reach significant level.