文献类型：期刊文献

英文题名：Transgenic Eucalyptus urophylla x Eucalyptus grandis superior clone DH3229: achieving glyphosate resistance through genetic modification

作者：Wang, Xuejun[1] Wei, Haoyu[1,2] Zhang, Shuang[1] Gao, Jiahui[1,2] Zeng, Bingshan[1] Fan, Chunjie[1]

第一作者：Wang, Xuejun

通信作者：Fan, CJ[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Key Lab State Forestry Adm Trop Forestry, Guangzhou 510520, Peoples R China;[2]Northeast Forestry Univ, Coll Forestry, Harbin 150040, Peoples R China

年份：2025

卷号：160

期号：2

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001412368400001)】；

基金：This work was supported by the National Key R&D Program of China during the 14th Five-year Plan Period (2021YFD2200102) and Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2020ZB004).

语种：英文

外文关键词：Eucalyptus; Genetic transformation; Herbicide; Weed control; EPSPS

摘要：Eucalyptus species are known for their high productivity and adaptability, making them a reliable source of timber. However, hybrid eucalyptus, particularly in the initial months of plantation, is susceptible to weed competition. Glyphosate is the most commonly used herbicide for weed control in eucalyptus plantations. To incorporate glyphosate resistance into eucalyptus cultivars (Eucalyptus urophylla x E. grandis DH3229), the g10-epsps gene, driven by the 35 S promoter, was introduced using an efficient Agrobacterium-mediated genetic transformation method. Following PCR-based selection and detection, six plants were confirmed to have successfully integrated the epsps gene into the eucalyptus genome at various stages. Realtime fluorescence quantitative PCR and enzyme-linked immunosorbent assay further validated the gene's integration and expression in all independent transformation events. Additionally, the transgenic plants demonstrated significant resistance to glyphosate after being treated with a 200-fold diluted Roundup solution. Physiological analysis revealed that maintaining lower levels of shikimic acid in transgenic plants is crucial for glyphosate tolerance. The successful development of herbicide-resistant transgenic eucalyptus in elite clones presents significant potential for future breeding programs and may be directly applicable to plantation after further field testing.