文献类型：期刊文献

英文题名：The Identification and Expression Analysis of the Nitraria sibirica Pall. Auxin-Response Factor (ARF) Gene Family

作者：Liu, Yuxin[1,2] Zhang, Jingbo[3] Li, Xinle[3] Zhu, Liming[1] Lian, Ziming[2] Fang, Hao[2] Lu, Lu[1] Lu, Ye[1] Shi, Jisen[1] Chen, Jinhui[1] Hao, Zhaodong[1] Cheng, Tielong[2]

第一作者：Liu, Yuxin

通信作者：Hao, ZD[1];Cheng, TL[2]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Minist Educ China, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Peoples R China;[2]Nanjing Forestry Univ, Coll Biol & Environm, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou 015200, Peoples R China

年份：2022

卷号：23

期号：19

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85139979037);WOS:【SCI-EXPANDED(收录号:WOS:000867760700001)】；

基金：This research was supported by the Youth Foundation of Nature Science Foundation of Jiangsu Province (BK20210614) and the Nature Science Foundation of China (31770715), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：auxin-response factors; drought stress; gene expression; Nitraria sibirica

摘要：Nitraria sibirica is a shrub that can survive in extreme drought environments. The auxin-response factors (ARFs) are a class of transcription factors that are widely involved in plant growth and development, as well as in the regulation of stress resistance. However, the genome-wide identification of the ARF gene family and its responses to environmental stresses, especially drought stress, in N. sibirica has not yet been reported. Here, we identified a total of 12 ARF genes in the genome of N. sibirica, which were distributed over 10 chromosomes and divided into three clades. Intragenome synteny analysis revealed one collinear gene pair in the ARF gene family, i.e., NsARF9a and NsARF9b. Cis-acting element analysis showed that multiple hormones and stress-responsive cis-acting elements were found in the promoters of NsARFs, suggesting that NsARFs may be involved in multiple biological processes. Quantitative real-time PCR (qRT-PCR) showed that many NsARFs had tissue-specific expression patterns, with the highest expression of NsARF16 in the seedlings of N. sibirica. In addition, most of the NsARFs that were upregulated under drought were independent of endogenous ABA biosynthesis, whereas the response of NsARF5 and NsARF7a to drought was disrupted by the ABA-biosynthesis inhibitor fluridone. These studies provide a basis for further research into how NsARFs in N. sibirica respond to hormonal signaling and environmental stresses.