文献类型：期刊文献

英文题名：Defensive alteration of root exudate composition by grafting Prunus sp. onto resistant rootstock contributes to reducing crown gall disease

作者：Chen, Lin[1] Bian, Lusen[1] Ma, Qinghua[1] Li, Ying[1] Wang, Xinghong[1] Liu, Yunpeng[2]

第一作者：陈淋

通信作者：Liu, YP[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Natl Permanent Sci Res Base Warm Temperate Zone Fo, Beijing 102300, Peoples R China;[2]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Beijing 100081, Peoples R China

年份：2024

卷号：11

期号：4

外文期刊名：HORTICULTURE RESEARCH

收录：;Scopus(收录号：2-s2.0-85190844914);WOS:【SCI-EXPANDED(收录号:WOS:001205650600001)】；

基金：The authors thank Lanying Liu and Jing Xu at the Laboratory of Plant Tissue Culture Technology of Haidian District for providing 'Haiying 1' seedlings. We thank Dr Dongwei Chen and Prof. Wen-bin Du (Chinese Academy of Sciences) for help in the chemotaxisassay. This work was supported by the National Natural Science Foundation of China (grants 32370135 and 31700548) and the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSAL-202302).

语种：英文

摘要：Grafting is a traditional and significant strategy to suppress soil-borne diseases, such as the crown gall disease caused by tumorigenic Agrobacterium and Rhizobium. Root exudates and the rhizosphere microbiome play critical roles in controlling crown gall disease, but their roles in suppressing crown gall disease in grafted plants remain unclear. Here, disease-susceptible cherry rootstock 'Gisela 6' and disease-resistant cherry rootstock 'Haiying 1' were grafted onto each other or self-grafted. The effect of their root exudates on the soil microbiome composition and the abundance of pathogenic Agrobacterium were studied. Grafting onto the disease-resistant rootstock helped to reduce the abundance of pathogenic Agrobacterium, accompanied by altering root exudation, enriching potential beneficial bacteria, and changing soil function. Then, the composition of the root exudates from grafted plants was analyzed and the potential compounds responsible for decreasing pathogenic Agrobacterium abundance were identified. Based on quantitative measurement of the concentrations of the compounds and testing the impacts of supplied pure chemicals on abundance and chemotaxis of pathogenic Agrobacterium and potential beneficial bacteria, the decreased valine in root exudates of the plant grafted onto resistant rootstock was found to contribute to decreasing Agrobacterium abundance, enriching some potential beneficial bacteria and suppressing crown gall disease. This study provides insights into the mechanism whereby grafted plants suppress soil-borne disease.