详细信息
PagERF81 regulates lignin biosynthesis and xylem cell differentiation in poplar ( SCI-EXPANDED收录) 被引量:7
文献类型:期刊文献
英文题名:PagERF81 regulates lignin biosynthesis and xylem cell differentiation in poplar
作者:Zhao, Xin-Wei[1,2] Wang, Qiao[2] Wang, Dian[3] Guo, Wei[4] Hu, Meng-Xuan[1] Liu, Ying-Li[1] Zhou, Gong-Ke[5] Chai, Guo-Hua[2] Zhao, Shu-Tang[1] Lu, Meng-Zhu[1,6]
第一作者:Zhao, Xin-Wei
通信作者:Zhao, ST[1];Lu, MZ[1];Lu, MZ[2]
机构:[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Qingdao Agr Univ, Coll Resources & Environm, Qingdao 266109, Peoples R China;[3]Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China;[4]Taishan Acad Forestry Sci, Tai An 271000, Peoples R China;[5]Qingdao Agr Univ, Coll Landscape Architecture & Forestry, Qingdao 266109, Peoples R China;[6]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China
年份:0
外文期刊名:JOURNAL OF INTEGRATIVE PLANT BIOLOGY
收录:;Scopus(收录号:2-s2.0-85150744342);WOS:【SCI-EXPANDED(收录号:WOS:000949285200001)】;
基金:ACKNOWLEDGEMENTS This work was supported by the National Key Scientific Research Project of China (2021YFD2200205) and the Ten-Thousand Talents Program of China to Meng-Zhu Lu.
语种:英文
外文关键词:lignin biosynthesis; PagERF81; Populus; secondary cell wall; xylem development
摘要:Lignin is a major component of plant cell walls and is essential for plant growth and development. Lignin biosynthesis is controlled by a hierarchical regulatory network involving multiple transcription factors. In this study, we showed that the gene encoding an APETALA 2/ethylene-responsive element binding factor (AP2/ERF) transcription factor, PagERF81, from poplar 84 K (Populus alba x P. glandulosa) is highly expressed in expanding secondary xylem cells. Two independent homozygous Pagerf81 mutant lines created by gene editing, produced significantly more but smaller vessel cells and longer fiber cells with more lignin in cell walls, while PagERF81 overexpression lines had less lignin, compared to non-transgenic controls. Transcriptome and reverse transcription quantitative PCR data revealed that multiple lignin biosynthesis genes including Cinnamoyl CoA reductase 1 (PagCCR1), Cinnamyl alcohol dehydrogenase 6 (PagCAD6), and 4-Coumarate-CoA ligase-like 9 (Pag4CLL9) were up-regulated in Pagerf81 mutants, but down-regulated in PagERF81 overexpression lines. In addition, a transient transactivation assay revealed that PagERF81 repressed the transcription of these three genes. Furthermore, yeast one hybrid and electrophoretic mobility shift assays showed that PagERF81 directly bound to a GCC sequence in the PagCCR1 promoter. No known vessel or fiber cell differentiation related genes were differentially expressed, so the smaller vessel cells and longer fiber cells observed in the Pagerf81 lines might be caused by abnormal lignin deposition in the secondary cell walls. This study provides insight into the regulation of lignin biosynthesis, and a molecular tool to engineer wood with high lignin content, which would contribute to the lignin-related chemical industry and carbon sequestration.
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