文献类型：期刊文献

英文题名：Transformation of Liquidambar formosana L. via Agrobacterium tumefaciens using a mannose selection system and recovery of salt tolerant lines

作者：Qiao, Guirong[1] Zhou, Jing[1] Jiang, Jing[1] Sun, Yuehua[1,2,4] Pan, Luanyin[1] Song, Honggai[1] Jiang, Jingmin[1] Zhuo, Renying[1] Wang, Xiaojuan[2] Sun, Zongxiu[3]

第一作者：乔桂荣

通信作者：Zhuo, RY[1]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, Key Lab Tree Genom, Res Inst Subtrop Forest, Hangzhou 311400, Zhejiang, Peoples R China;[2]Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Gansu, Peoples R China;[3]China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China;[4]Gansu Polytech Coll Anim Husb & Engn, Wuwei 733006, Gansu, Peoples R China

年份：2010

卷号：102

期号：2

起止页码：163-170

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;EI(收录号：20102613046442);Scopus(收录号：2-s2.0-77954028496);WOS:【SCI-EXPANDED(收录号:WOS:000279154600004)】；

基金：The authors thank Dr. Roberto A. Gaxiola at the University of Connecticut for kindly providing AtNHX1 cDNA. This work was supported by the 948 Program (No.2005-4-58, No.2006-4-C01) of the State Forestry Administration People's Republic of China and the Natural Science Foundation of Zhejiang Province (Y306072).

语种：英文

外文关键词：Liquidambar formosana L.; Transformation; Mannose-selection marker; AtNHX1

摘要：A mannose selection system was adapted for use in the Agrobacterium-mediated transformation of Liquidambar formosana L. This system makes use of the pmi gene, which encodes phosphomannose isomerase. This protein converts mannose-6-phosphate to fructose-6-phosphate. Leaf explants from an axenic plant of L. formosana L. were infected with Agrobacterium. The gene AtNHXI was transformed into L. formosana L. using the transformation procedure developed in this study. We found that supplementing the media with 1.5% (w/v) mannose and 1% sucrose provided the necessary conditions for the selection of transformed plants from non-transformed plants. In this study, 134 positive transgenic plants were obtained. Genetic transformation was confirmed by PCR and Southern blot analysis, while RT-PCR confirmed expression of the foreign gene (AtNHX1) in the regenerated plants. Chlorophenol red assays confirmed the activity of transgenes in regenerated plants. In order to ascertain the tolerance of transformants for salt, further experimentation was done. Results showed that the soluble protein content in three transgenic lines (T-12, T-22, T-43) was higher than that in the control plant, while the MDA content was lower. Our results show that transgenic L. formosana L. plants can be obtained using either antibiotic resistance genes that are not expressed in the microorganisms or an antibiotic-free positive selection system.