文献类型：期刊文献

英文题名：Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa

作者：Lin, Ying-Chung Jimmy[1,2,3] Chen, Hao[4] Li, Quanzi[5] Li, Wei[1,4] Wang, Jack P.[1,4] Shi, Rui[6] Tunlaya-Anukit, Sermsawat[4] Shuai, Peng[4,7] Wang, Zhifeng[1] Ma, Hongyan[1] Li, Huiyu[1,4] Sun, Ying-Hsuan[8] Sederoff, Ronald R.[4] Chiang, Vincent L.[1,4]

第一作者：Lin, Ying-Chung Jimmy

通信作者：Lin, YCJ[1];Chiang, VL[1];Lin, YCJ[2];Lin, YCJ[3];Chiang, VL[4]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[2]Natl Taiwan Univ, Dept Life Sci, Coll Life Sci, Taipei 10617, Taiwan;[3]Natl Taiwan Univ, Coll Life Sci, Inst Plant Biol, Taipei 10617, Taiwan;[4]North Carolina State Univ, Forest Biotechnol Grp, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA;[5]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China;[6]North Carolina State Univ, Crop & Soil Sci Dept, Raleigh, NC 27695 USA;[7]Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou 350002, Fujian, Peoples R China;[8]Natl Chung Hsing Univ, Dept Forestry, Taichung 40227, Taiwan

年份：2017

卷号：114

期号：45

起止页码：E9722-E9729

外文期刊名：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000414631200035)】；

基金：We thank the National Natural Science Foundation of China (NSFC) for financial support from Grants 31430093, 31522014, 31370593, and 31670674. This work was also supported by US Office of Science (Biological and Environmental Research), Department of Energy Grant DE-SC000691 and the North Carolina State University Jordan Family Distinguished Professor Endowment.

语种：英文

外文关键词：reciprocal cross-regulation; NAC transcription factors; alternative splicing; wood formation; Populus trichocarpa

摘要：Secondary cell wall (SCW) biosynthesis is the biological process that generates wood, an important renewable feedstock for materials and energy. NAC domain transcription factors, particularly VascularRelated NAC-Domain (VND) and Secondary Wall-Associated NAC Domain (SND) proteins, are known to regulate SCW differentiation. The regulation of VND and SND is important to maintain homeostasis for plants to avoid abnormal growth and development. We previously identified a splice variant, PtrSND1-A2(IR), derived from PtrSND1-A2 as a dominant-negative regulator, which suppresses the transactivation of all PtrSND1 family members. PtrSND1-A2IR also suppresses the self-activation of the PtrSND1 family members except for its cognate transcription factor, PtrSND1-A2, suggesting the existence of an unknown factor needed to regulate PtrSND1-A2. Here, a splice variant, PtrVND6-C1(IR), derived from PtrVND6-C1 was discovered that suppresses the protein functions of all PtrVND6 family members. PtrVND6-C1(IR) also suppresses the expression of all PtrSND1 members, including PtrSND1-A2, demonstrating that PtrVND6-C1(IR) is the previously unidentified regulator of PtrSND1A2. We also found that PtrVND6-C1(IR) cannot suppress the expression of its cognate transcription factor, PtrVND6-C1. PtrVND6-C1 is suppressed by PtrSND1-A2(IR). Both PtrVND6-C1(IR) and PtrSND1-A2(IR) cannot suppress their cognate transcription factors but can suppress all members of the other family. The results indicate that the splice variants from the PtrVND6 and PtrSND1 family may exert reciprocal cross-regulation for complete transcriptional regulation of these two families in wood formation. This reciprocal cross-regulation between families suggests a general mechanism among NAC domain proteins and likely other transcription factors, where intronretained splice variants provide an additional level of regulation.