文献类型：期刊文献

中文题名：空间环境对草地早熟禾叶片解剖结构及同工酶酶谱的影响

英文题名：The Effect of the Space Condition on the Anatomical Characters of the Leaves and the Ioszyme of Peroxidase and Esterase in Poa pratensis

作者：韩蕾[1] 孙振元[1] 郭燕[2] 佟一超[3] 李银凤[1] 彭镇华[1]

第一作者：韩蕾

机构：[1]中国林业科学研究院生物技术室;[2]华中农业大学;[3]北京飞鹰绿色食品有限公司

年份：2004

卷号：17

期号：3

起止页码：310-315

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：优质抗逆草坪草早熟禾等新品种选育(国家863计划,2002AA241061)

语种：中文

中文关键词：空间环境;“神舟”三号搭载;草地早熟禾;解剖结构;同工酶

外文关键词：space condition;Poa pratensis;anatomical characters;peroxidase;esterase;ioszyme

分类号：S731.2

摘要：将"神舟"三号(SZ 3)飞船搭载的草地早熟禾品种纳苏干种子地面种植后,根据形态变化进行初步筛选,并通过分株扩繁出突变株系PM1、PM2和PM3。对这三个草地早熟禾突变株系的叶片解剖结构及过氧化物酶同工酶和酯酶同工酶进行观察。结果表明,与对照(CK)相比,三个变异株系由于细胞数目的增加使叶片宽度明显增加;叶肉细胞平均直径降低使叶片厚度减少;PM2、PM3株系叶片泡状细胞的增多,改变了叶片的形态。过氧化物酶同工酶谱带CK有11条,迁移率为4 63%～47 22%;PM1有21条谱带,迁移率为2 78%～68 52%;PM2有18条谱带,迁移率为2 78%～67 59%;PM3有20条谱带,迁移率为2 78%～63 89%。酯酶同工酶谱带CK有3条,迁移率为59 74%～94 81%;PM1有8条谱带,迁移率为38 96%～94 81%;PM2有7条谱带,迁移率为48 05%～94 81%;PM3有8条谱带,迁移率为49 35%～94 81%。PM1、PM2和PM3三个株系与CK含有一部分完全相同的谱带,另一部分谱带发生了较大变化:分别出现了CK中没有的谱带和在CK中存在而三个变异株系没有的谱带。同时各谱带的强弱也发生了不同程度的变化。
Taking the dry seeds of Poa pratensis Nassau carried by SZ-3 as the treatment and the seeds which on the earth as CK,three mutations were screened based on presentational characters from the plants treatment and asexual reproduction as PM_1,PM_2 and PM_3.The effect of the space condition on the anatomical characters of the leaves and the ioszyme of peroxidase and esterase in leaves were observed and investigated.The results showed that compared to CK,the width of the leaves of the three mutations increased as the number of epidermis increased,the thickness of the leaves reduced as the average diameter of the mesophyllous cells decreased.The number of the bulliform cells in the leaves of PM_2 and PM_3 increased which changed the shape of the leaves.The results of the ioszyme of esterase showed that there were 11 bands in CK and the Rf value was 4.63%～47.22%;21 bands in PM_1 and Rf value was 2.78%～68.52%;18 bands in PM_2 and Rf value was 2.78%～67.59%;20 bands in PM_3 and Rf value was 2.78%～63.89%.The results of the ioszyme of peroxidase showed that there were bands in CK and the Rf value was 59.74%～94.81%;8 bands in PM_1 and the Rf value was 38.96%～94.81%;7 bands in PM_2 and the Rf value was 48.05%～94.81%;8 bands in PM_3 and the Rf value was 49.35%～94.81%.There were some same bands both in CK and the mutations,but there were also some other bands changed:some of them existed in the mutations but not in CK;some of them existed in CK but not in the mutations;and the activities of the ioszyme were changed greatly.All of these mutations were valuable to production application and genetic breeding.