文献类型：期刊文献

英文题名：Assembly and analysis of the complete mitochondrial genome of endangered plant Tilia amurensis Rupr

作者：Liu, Xiao[1] Lin, Furong[2] Zhai, Shasha[3] Wu, Zhicheng[3] Gao, Lei[3] Sun, Nana[4] Yan, Wenchao[5] Chen, Jitong[4] Liu, Dan[3]

第一作者：Liu, Xiao

通信作者：Liu, D[1]

机构：[1]Qufu Normal Univ, Jining, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry, Beijing, Peoples R China;[3]Shandong Prov Ctr Forest & Grass Germplasm Resourc, Beijing, Peoples R China;[4]Shandong Normal Univ, Jinan, Peoples R China;[5]Hebei Normal Univ Sci & Technol, Qinhuangdao, Peoples R China

年份：2025

卷号：16

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-105025523119);WOS:【SCI-EXPANDED(收录号:WOS:001640379600001)】；

基金：The author(s) declare financial support was received for the research and/or publication of this article. This study was funded by the National Forestry and Grassland Germplasm Resources Platform (2005DKA21003) and Key R&D Program of Shandong Province (2024LZGC003).

语种：英文

外文关键词：Tilia amurensis Rupr.; endangered plant; mitochondrial genome; repetitive sequence; chloroplast-derived sequence

摘要：Introduction Tilia amurensis Rupr., a deciduous tree of the genus Tilia in the Malvaceae family, is classified as a second-class key protected wild plant in China. This study aimed to sequence and analyze the complete mitochondrial genome of T. amurensis to (i) aid the understanding of phylogenetic relationships within the Malvaceae family, (ii) clarify the taxonomic status of genus Tilia, and (iii) provide a molecular basis for the conservation and genetic breeding of this endangered species.Methods T. amurensis mitochondrial genome was sequenced and assembled using the Illumina and Oxford Nanopore Technologies platforms. Additionally, bioinformatics analyses were conducted, along with gene annotation and functional analysis, to identify repeat sequences and chloroplast-derived fragments.Results The assembled mitochondrial genome of T. amurensis was found to be a single circular molecule with a total length of 830,088 bp and a GC content of 44.97%. A total of 61 genes were annotated, including 20 tRNA, 3 rRNA, and 38 protein-coding genes. Moreover, 472 RNA editing sites were identified among the protein-coding genes. We also detected a high abundance of repetitive sequences and chloroplast-derived fragments. Specifically, the genome included 264 simple sequence repeats, 19 tandem repeats, and 855 pairs of scattered repeats, predominantly comprising short fragment repetitions. Furthermore, 22 chloroplast homologous fragments (with a cumulative length of 8,138 bp) were identified, accounting for 0.98% of the total mitochondrial genome length. Phylogenetic analysis based on the mitochondrial genomes of 35 plant species confirmed that T. amurensis, within the Malvaceae family of the Malvales order, is consistent with the latest classification proposed by the Angiosperm Phylogeny Group.Discussion This study reports, for the first time, the complete mitochondrial genome sequence of T. amurensis, revealing its high reproducibility and notable chloroplast-to-mitochondria DNA transfer. These findings provide valuable data for understanding the evolutionary dynamics of the Malvaceae mitochondrial genome. Furthermore, they establish a molecular foundation for future research on the endangerment mechanisms of T. amurensis, its genetic diversity, and development of effective conservation strategies.