文献类型：期刊文献

中文题名：西伯利亚白刺基因组信息初探

英文题名：A study on Nitraria sibirica Pall Genome

作者：朱礼明[1,2] 黎梦娟[1,2] 张景波[3] 杨秀艳[3,4] 成铁龙[1,2]

第一作者：朱礼明

机构：[1]南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037;[2]南京林业大学南方现代林业协同创新中心,江苏南京210037;[3]中国林业科学研究院沙漠林业实验中心,内蒙古磴口015200;[4]中国林业科学研究院国家林业和草原局盐碱地研究中心,北京100091

年份：2020

卷号：33

期号：1

起止页码：144-151

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：唐古特白刺SOS信号通路在耐受高盐胁迫中的作用及其表观遗传分析(31770715);

语种：中文

中文关键词：西伯利亚白刺;流式细胞术;基因组大小;GC含量;SSR标记

外文关键词：Nitraria sibirica Pall;Flow cytometry;genome size;GC content;SSR molecular marker

分类号：S722

摘要：[目的]为了解西伯利亚白刺(Nitraria sibirica Pall)基本基因组信息,如基因组大小、复杂程度等特征。[方法](1)以西伯利亚白刺的幼苗嫩叶为材料,以番茄为标定,用解离液mG解离叶片后,经流式细胞仪检测细胞悬液,收集细胞并计算西伯利亚白刺的C值。(2)通过构建小片段文库,进行Illumina HiSeq测序并分析测序结果,预估其基因组大小、GC含量、杂合率、重复序列比例等,使用MISA脚本对过滤后数据进行SSR位点分析。[结果]西伯利亚白刺预估全基因组大小为526.30 Mbp,基因组GC含量为36.78%,基因组杂合率为0.90%,重复序列比例为55.39%.对测序数据进行SSR位点标记,共得到521125个SSR位点,其中,单核昔酸重复为342883个,占总SSR位点的65.80%。[结论]由各项分析指标推测西伯利亚白刺为复杂基因组,可以采用二代Illumina与三代PacBio测序辅以Hi-C技术联合分析,有利于西伯利亚白刺基因组的组装及基因图谱的获取。
[Objective]This study aims at estimating the size,complexity and other characteristics of Nitraria sibirica Pall genome.[Method]Taking Solanum lycopersicum as DNA reference standard,the young leaves of N sibirica were used as test material and mG as separate buffer to establish the N.sibirica genomic C-value by flow cytometry(FCM)method.A small fragment library was established for Illumina HiSeq sequencing.The genome size,heterozygosity,GC content and repetitive sequence ratio were studied and MISA script was used to analyze the SSR data.[Result]The size of N.sibirica genome was 526.30 Mbp,the heterozygosity rate was 0.90%,the GC content ratio was 36.78%and the repeat sequence ratio was 55.39%?Using the assembled genome data for SSR analysis obtained 521125 loci,of which mononucleotide repeat were 342883 loci,accounting for 65.80%of the total SSRs.[Conclusion]It is predicted that the N.sibirica genome has a complex nature and can be analyzed via Illumina combined to PacBio and Hi-C technologies in order to facilitate the assemble of N.sibirica genome and the acquisition of genetic maps.