文献类型：期刊文献

英文题名：Identification, characterization, and expression of the SWEET gene family in Phalaenopsis equestris and Dendrobium officinale

作者：Wang, T.[1] Song, Z.[1] Meng, W. L.[2] Li, L. B.[1]

第一作者：Wang, T.

通信作者：Li, LB[1]

机构：[1]Chinese Acad Forestry, State Key Lab Forest Genet & Tree Breeding, Key Lab Silviculture State Forestry Adm, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Environm Protect Res Inst Handan, Handan 056002, Peoples R China

年份：2018

卷号：62

期号：1

起止页码：24-32

外文期刊名：BIOLOGIA PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000426293900003)】；

基金：This work was supported by grants from the Ministry of Science and Technology of China (No. 2012BAD01B0702 and No. 2013AA102607).

语种：英文

外文关键词：floral organs; high or low temperature; orchids; phylogenetic trees; sugar transporters

摘要：Sugars are important molecules that function not only as primary metabolites, but also as nutrients and signal molecules in plants. The sugar transport protein genes family SWEET has been recently identified. The availability of the Dendrobium officinale and Phalaenopsis equestris genome sequences offered the opportunity to study the SWEET gene family in this two orchid species. We identified 22 and 16 putative SWEET genes, respectively, in the genomes of D. officinale and P. equestris using comprehensive bioinformatics analysis. Based on phylogenetic comparisons with SWEET proteins from Arabidopsis and rice, the DoSWEET and PeSWEET proteins could be divided into four clades; among these, clade II specifically lacked PeSWEETs and clade IV specifically lacked DoSWEETs, and there were orthologs present between D. officinale and P. equestris. Protein sequence alignments suggest that there is a predicted serine phosphorylation site in each of the highly conserved MtN3/saliva domain regions. Gene expression analysis in four tissues showed that three PeSWEET genes were most highly expressed in the flower, leaf, stem, and root, suggesting that these genes might play important roles in growth and development in P. equestris. Analysis of gene expression in different floral organs showed that five PeSWEET genes were highly expressed in the column (gynostemium), implying their possible involvement in reproductive development in this species. The expression patterns of seven PeSWEETs in response to different abiotic stresses showed that three genes were upregulated significantly in response to high temperature and two genes were differently expressed at low temperature. The results of this study lay the foundation for further functional analysis of SWEET genes in orchids.