文献类型：期刊文献

中文题名：丽蝇蛹集金小蜂气味结合蛋白基因的克隆与序列分析

英文题名：Molecular cloning and sequence analysis of odorant binding protein gene from Nasonia vitripennis

作者：朱家颖[1] 杨璞[2] 吴国星[3] 和秋菊[1] 尹立红[1] 熊忠平[1]

第一作者：朱家颖

机构：[1]西南林业大学云南省森林灾害预警与控制重点实验室;[2]中国林业科学研究院资源昆虫研究所;[3]云南农业大学植物保护学院

年份：2010

卷号：32

期号：4

起止页码：476-482

中文期刊名：环境昆虫学报

外文期刊名：Journal of Environmental Entomology

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：云南省教育厅科学研究基金项目(2010Y294);西南林学院重点科研基金项目(110903);云南省森林灾害预警与控制重点实验室开放基金项目(ZK09A101);云南省重点学科森林保护学资助项目(XKZ200905)

语种：中文

中文关键词：丽蝇蛹集金小蜂;气味结合蛋白;基因克隆;序列分析;组织表达

外文关键词：Nasonia vitripennis; odorant binding protein; gene clone; sequence analysis; tissue expression

分类号：Q966

摘要：利用RACE技术,首次从丽蝇蛹集金小蜂Nasonia vitripennis中克隆获得一个气味结合蛋白全长cDNA序列。该基因全长553bp,开放阅读框411bp,3′和5′端非编码序列分别为13bp和129bp。其推导的氨基酸序列编码136个氨基酸,推测编码蛋白质的分子量为15.4kDa,等电点为8.76。同源性比对分析发现,丽蝇蛹集金小蜂气味结合蛋白基因与现已报道的其它昆虫气味结合蛋白基因在氨基酸水平上的相似性均低于30%,拥有6个保守半胱氨酸位点等气味结合蛋白所具有的典型特征。系统进化树分析表明,丽蝇蛹集金小蜂气味结合蛋白与意大利蜜蜂Apismellifera气味结合蛋白2,3,4,5,6,7,8和12聚为同一族,与意大利蜜蜂气味结合蛋白5,6和8的进化程度最近。RT-PCR分析表明,丽蝇蛹集金小蜂气味结合蛋白基因不仅在雌、雄成虫触角中高度表达,而且在头部和足中有微弱表达。
In this paper,a cDNA clone coding odorant binding protein was first isolated from Nasonia vitripennis using rapid amplification of cDNA ends （RACE） strategy. This gene was 411 bp in full-length with open reading frame （ORF） of 411 bp,containing 3＇ and 5＇ untranslated region of 13 bp and 129 bp. The deduced amino acid sequence of N. vitripennis odorant binding protein revealed mature proteins of 136 amino acids. Its predicted molecular weight and isoelectric point were 15.4 kD and 8.76,respectively. N. vitripennis odorant binding protein shared low （below 30%） amino acid identity with other insects＇ odorant binding protein,and typical structural features of odorant binding proteins,e.g.,including the six conserved cysteine motif. Phylogenetic tree indicated that N. vitripennis odorant binding protein was fall into the same clade with Apis mellifera odorant binding protein 2,3,4,5,6,7,8 and 12,and has closest relationship with A. mellifera odorant binding protein 5,6 and 8. RT-PCR analyses showed that N. vitripennis odorant binding protein mRNA was highly expressed in the adult antennae of both females and males of both sexes,and weakly detected in the head and leg.