文献类型：期刊文献

中文题名：大花序桉SSR位点多样性和群体结构分析

英文题名：Analysis of SSR Marker Diversity and Population Structure in Eucalyptus cloeziana

作者：王莉[1,2] 李昌荣[3] 李发根[1,2] 周长品[1,2] 翁启杰[1] 李梅[1] 吕佳斌[1] 陈健波[3] 陈剑成[4] 项东云[3] 甘四明[1,2,3]

第一作者：王莉

机构：[1]中国林业科学研究院热带林业研究所,热带林业研究国家林业局重点实验室,广州510520;[2]中国林业科学研究院,林木遗传育种国家重点实验室,北京100093;[3]广西壮族自治区林业科学研究院,广西优良用材林资源培育重点实验室,中南速生材繁育国家林业局重点实验室,南宁530002;[4]玉林市林业科学研究所,容县3537501

年份：2019

卷号：17

期号：13

起止页码：4470-4478

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：广西创新驱动发展专项(桂科AA17204087-3);“广西主要用材林资源高效培育与利用人才小高地”专项(桂财社函[2018]112)共同资助

语种：中文

中文关键词：大花序桉;SSR;遗传多样性;群体结构

外文关键词：Eucalyptus cloeziana;SSR;Genetic diversity;Population structure

分类号：S565.1

摘要：为了获得大花序桉群体变异的可靠信息,本研究基于桉树29个基因组简单序列重复(SSR)标记,筛选了既不偏离哈温平衡、又非群体间分化系数(Fst)离群值的中性SSR用于大花序桉14个群体的遗传多样性和群体结构分析。基于11个中性SSR位点,群体的每位点等位片段数(Na)和特有等位片段数(Npa)平均分别为6.88和4.5,观测杂合度(Ho)和期望杂合度(He)平均分别为0.749和0.780,证明群体多样性较高;固定化指数(F)平均为0.040,表明群体内近交水平极低。位点平均Fst为0.120,分子方差分析(AMOVA)中群体间变异只占总方差分量的3.1%,表明群体为中等到低的分化水平。主坐标分析、聚类分析和群体结构分析都将昆士兰州北部与中部群体(各7个)分为不同的亚群体。这为大花序桉种质资源保存和育种策略制定提供了理论的参考依据。
In order to obtain reliable information on population variation in Eucalyptus cloeziana F. Meull., 11 neutral simple sequence repeats(SSR) markers that neither showed between-population differentiation(Fst) outliers nor departed from Hardy-Weinberg equilibrium were screened out from 29 genomic SSRs for analyses of genetic diversity and population structure of the species. Based on the 11 neutral SSR loci, the number of alleles(Na), the number of private alleles(Npa), observed heterozygosity(Ho) and expected heterozygosity(He) were av eraged at 6.88, 4.5, 0.749 and 0.780, respectively, demonstrating high levels of genetic diversity. Mean fixation index(F) was 0.040, indicating a very low degree of inbreeding. Overall Fstwas 0.120 and analysis of molecular variance(AMOVA) showed that among-population variance took only 3.1% of the total variance, suggesting moderate to relatively low levels of population differentiation. Principal coordinate analysis, cluster analysis and structure analysis resulted consistently in obvious separation of the seven northern populations from the seven middle populations from Queensland. The results will be helpful for germplasm conservation and breeding strategy making in E. cloeziana.