文献类型：期刊文献

英文题名：Development of simple sequence repeat (SSR) markers and genetic diversity analysis in black-wood (Acacia melanoxylon) clones in china

作者：Fan, Chunjie[1] Liu, Qianyu[1] Zeng, Bingshan[1] Qiu, Zhenfei[1] Zhou, Changpin[1] Chen, Kaoke[1] Guo, Guangsheng[1]

第一作者：范春节

通信作者：Zeng, BS[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2016

卷号：65

期号：1

起止页码：49-54

外文期刊名：SILVAE GENETICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000406712600006)】；

基金：We would like to thank the Forestry Administration of Guangdong Province for financially supporting this study through the Forestry Technology Innovation Program of Guangdong grant (2014KJCX010).

语种：英文

外文关键词：Acacia melanoxylon; SSR; genetic diversity; polymorphism information content

摘要：Understanding the genetic diversity of Acacia melanoxylon is very important in species selection and improvement. The present study aimed to identify microsatellite markers and determine the genetic diversity of 45 preferred clones selected from 9 Chinese districts. Seventy-six simple sequence repeat (SSR) markers developed for other Acacia species were screened. Seventeen SSR markers showed polymorphic patterns and amplified 134 alleles. Polymorphism information content (PIC) values ranged from 0.044 to 0.911. The average allele number per locus ranged between 2 and 18, averaging 8.06. Nine SSR markers were highly informative, with PIC values above 0.60. These findings demonstrated that SSR markers could be used to differentiate A. melanoxylon genotypes. Cluster analysis using UPGMA separated the 45 clones into 3 distinct groups at a similarity coefficient of 0.72. The clone groups identified in this study would be useful in developing intraspecific hybrids to exploit hybrid vigor as well as for commercial cultivation and genetic base broadening. The DNA fingerprints obtained for each clone could be used for biodiversity conservation.