文献类型：期刊文献

英文题名：Genome-Wide Identification of m(6)A Writers, Erasers and Readers in Poplar 84K

作者：Sun, Xiaochen[1] Wu, Wenli[1] Yang, Yanfang[1] Wilson, Iain[2] Shao, Fenjuan[1] Qiu, Deyou[1]

第一作者：Sun, Xiaochen

通信作者：Shao, FJ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, State Key Lab Tree Genet & Breeding,Key Lab Tree, Beijing 100091, Peoples R China;[2]CSIRO Agr & Food, Canberra, ACT 2601, Australia

年份：2022

卷号：13

期号：6

外文期刊名：GENES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000816109600001)】；

基金：This research was supported by the Natural Science Foundation of China (grant numbers 32171809 to D.Q.).

语种：英文

外文关键词：m(6)A modification; writers; erasers; readers; LBD 15; poplar 84K

摘要：N-6-methyladenosine (m(6)A) RNA modification is a conserved mechanism to regulate gene expression that plays vital roles in the development of plants. However, the m(6)A RNA modification in forest trees remains limited. Here, we performed a complete analysis of m(6)A writers, erasers and readers in Poplar 84K, including gene location, gene structures, conserved motifs, phylogenetic relationships, promoter analysis, expression profiles and the homology modeling. We have identified 61 m(6)A pathway genes in Poplar 84K (Populus alba x Populus glandulosa), including 14 m(6)A writers, 14 m(6)A erasers and 33 m(6)A readers. Phylogenetic analysis indicated that the m(6)A writers and erasers were clustered into four groups and m(6)A readers were clustered into two groups. Promoter analysis showed that m(6)A pathway genes were mainly responsive to low oxygen followed by ABA and ethylene. The expression of the identified m(6)A pathway genes showed tissue-specific expression patterns in leaves, xylem, phloem and roots. Moreover, 17 genes were significantly up-regulated and 13 genes were significantly down-regulated in poplar overexpressing the transcription factor LBD15. Homology modeling and molecular docking results suggested that PagFIP37b was most likely to be regulated by LBD15, and the qPCRshowed that PagFIP37b were up-regulated in the LBD15-oe plants. The results provide insights that aid in the future elucidation of the functions of these m(6)A pathway genes and the epigenetic regulation mechanism of these genes in Poplar 84K.