文献类型：期刊文献

中文题名：基质固相分散和HPLC-IT-TOF/MS联用研究漆树木材中多酚成分

英文题名：Study on Polyphenols in Lacquer Tree by Matrix Solid-phase Dispersion and HPLC-IT-TOF/MS

作者：陈虹霞[1] 周昊[1,2] 陶冉[1] 齐志文[1] 李文君[1] 王成章[1,2]

第一作者：陈虹霞

机构：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点实验室,江苏省生物质能源与材料重点实验室,江苏省林业资源高效加工利用协同创新中心,江苏南京210042;[2]中国林业科学研究院林业新技术研究所,北京100091

年份：2022

卷号：42

期号：1

起止页码：10-20

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划资助项目(2017YFD0601305);江苏省生物质能源与材料重点实验室开放基金资助项目(JSBEM-S-201908)。

语种：中文

中文关键词：基质固相分散;漆树;多酚;高效液相色谱-离子阱-飞行时间质谱

外文关键词：matrix solid-phase dispersion;lacquer tree;polyphenols;HPLC-ESI-TOF-MS

分类号：TQ351

摘要：采用基质固相分散(MSPD)技术进行漆树木中多酚化合物的提取,根据单因素试验得到优化工艺条件为:ZSM-22为吸附基质,吸附基质与样品的质量比为2∶1,洗脱溶剂为体积分数70%甲醇,洗脱溶剂用量8 mL,研磨时间2 min。在此条件下,漆树木多酚提取得率为(466±2.05)mg/g(以没食子酸计)。采用高效液相色谱-离子阱-飞行时间质谱(HPLC-IT/TOF-MS)联用技术,建立高效、准确鉴定漆树木中多酚化学成分的方法,根据质谱图和裂解规律信息,并结合文献,共鉴定10个化合物,包括3个没食子酸(GAC)类化合物和7个黄酮类化合物,分别是没食子酸、没食子酸甲酯、没食子酸乙酯、黄颜木素、花旗松素、3,4′,7-三羟基黄烷酮、异鼠李素、槲皮苷、漆黄素和硫黄菊素。利用基质固相分散和HPLC-IT/TOF-MS技术有利于漆树木中化合物的发现和鉴别,对漆树提取物的质量评价和后续分析具有重要的意义。
Matrix solid-phase dispersion was proposed to extract polyphenols in Toxicodendron vernicifluum(Stokes)F.A.Barkl.(Shaanxi lacquer tree).The MSPD conditions to extract polyphenols established by the single factor test were ZSM-22 as the adsorbent,adsorbent/sample ratio of 2∶1(g∶g),grinding for 2 min,and use of 8 mL of 70%methanol as the elution solvent.Under these conditions,the polyphenols content of MSPD extraction was(466±2.05)mg/g(calculated by gallic acid).An efficient and accurate method for identification of polyphenols in Shaanxi lacquer tree was established by HPLC-IT-TOF/MS.According to mass spectrometry,fragmentation pathway combined with published reports,total of 10 components were identified,including 3 gallic acid derivatives and 7 flavonoids as follows:gallic acid,methyl gallate,ethyl gallate,fustin,taxifolin,garbanzol,isorhamnetin,quercitrin,fisetin,sulfuretin.It was beneficial to the discovery and identification of compounds in Shaanxi lacquer tree by matrix solid-phase dispersion coupled with HPLC-IT-TOF/MS,and it could be significance for the analysis and quantification of extracts from Shaanxi lacquer tree samples.