详细信息
漆树籽粕多糖的提取工艺优化及抗氧化活性研究 被引量:2
Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity
文献类型:期刊文献
中文题名:漆树籽粕多糖的提取工艺优化及抗氧化活性研究
英文题名:Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity
作者:陈虹霞[1] 王成章[1,2] 叶建中[1] 周昊[1,2] 陶冉[1] 李文君[1]
第一作者:陈虹霞
机构:[1]中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室;[2]中国林业科学研究院林业新技术研究所
年份:2016
卷号:37
期号:4
起止页码:254-258
中文期刊名:食品工业科技
外文期刊名:Science and Technology of Food Industry
收录:CSTPCD;;北大核心:【北大核心2014】;CSCD:【CSCD_E2015_2016】;
基金:国家林业局948技术引进项目(2015-4-46);中国林科院林业新技术所基本科研业务费专项资金(CAFINT2013C03);国家国际科技合作专项(2014DFR31300)
语种:中文
中文关键词:漆籽;籽粕;多糖;提取;抗氧化活性
外文关键词:Rhus succedanea L; seed cakes; polysaccharides; extraction; antioxidant activity
分类号:TS255.1
摘要:以脱脂后的漆树籽粕为原料,采用热回流提取漆树籽粕多糖,通过单因素实验和正交实验优化该方法的最佳工艺条件,并进一步研究漆树籽粕多糖对羟自由基和ABTS+自由基的清除能力。结果表明:最佳工艺条件为提取时间2.5 h,料液比1∶20(g∶m L),提取温度80℃和提取次数2次,此时籽粕多糖的得率为1.561%。漆树籽粕多糖对清除羟自由基和ABTS+自由基的IC_(50)分别为8.515 mg·m L^(-1)和7.03 mg·m L^(-1),当漆树籽粕多糖的浓度为25 mg·m L^(-1)时,对羟自由基的清除率达到61.5%,当浓度为10 mg·m L-1时,对ABTS+自由基的清除率达到58.4%。体外抗氧化实验表明漆树籽粕多糖抗氧化活性作用明显。
Single factor and orthogonal methodology were used to optimize the extraction conditions of crude polysaccharides from the seed cakes of Rhus succedanea L,then the antioxidant(scavenging hydroxyl and ABTS radicals) of the crude polysaccharides from the seed cakes of Rhus succedanea L were possessed. The optimum extraction conditions were as follows :extraction time 2.5 h,ratio of material to water 1 ∶20(g∶m L),extraction temperature 80 ℃,and extraction times 2. Under the optimized conditions,an experimental yield was 1. 561 %. The IC_50 values scavenging hydroxyl and ABTS^+ radicals of the crude polysaccharides were8.515 mg·m L^-1 and 7.03 mg·m L^-1,respectively. The hydroxyl radical scavenging ability of polysaccharides reached 61.5% at 25 mg·m L^-1. The ABTS+radicals scavenging ability of polysaccharides reached 58.4% at10 mg·m L^-1. The seed cakes polysaccharides displayed significant antioxidant.
参考文献:
正在载入数据...