文献类型：期刊文献

英文题名：Whole transcriptome analysis of three leaf stages in two cultivars and one of their F-1 hybrid of Camellia sinensis L. with differing EGCG content

作者：Lin, Jinke[1] Wilson, Iain W.[2] Ge, Guoping[1,3] Sun, Guiling[4] Xie, Fuliang[5] Yang, Yanfang[6] Wu, Liangyu[1] Zhang, Baohong[5] Wu, Jianqiang[4] Zhang, Yue[1] Qiu, Deyou[6]

第一作者：Lin, Jinke

通信作者：Qiu, DY[1]|[a000598d4c276c5f9aea6]邱德有;

机构：[1]Fujian Agr & Forestry Univ, Anxi Coll Tea Sci, Dept Tea Sci, Fuzhou, Peoples R China;[2]CSIRO Agr & Food, Canberra, ACT, Australia;[3]Agr Extens Serv Ctr, Suichang, Zejiang, Peoples R China;[4]Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming, Peoples R China;[5]East Carolina Univ, Dept Biol, Greenville, NC USA;[6]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2017

卷号：13

期号：1

外文期刊名：TREE GENETICS & GENOMES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000397239600013)】；

基金：This research was financially supported by the grants from the National Natural Science Foundation of China (grant no. 31170651). We thank Dr. Liangsheng Zhang for technical assistance and Li Zhang for critical reading of the manuscript.

语种：英文

外文关键词：Camellia sinensis; Tea plant; Transcriptome; Leaf; Epigallocatechin-3-gallate; Gene; Expression regulation

摘要：Tea (Camellia sinensis (L.) O. Kuntze) is one of the most beneficial beverages due to its health attributes. Whole leaf transcriptome analysis of three leaf stages of C. sinensis has the potential to reveal candidate genes associated with these major beneficial compounds. Epigallocatechin-3-gallate (EGCG) is one of the most important bioactive catechin (antioxidant) in tea drink derived. EGCG has many important physiological functions and positive effects on human health, such as anticancer, antioxidation, controlling blood pressure, and blood sugar content. Although the major metabolic pathways of tea-specific compounds have been established, knowledge of the genes involved in the regulation and synthesis of EGCG in C. sinensis is limited. To gain insight into the molecular mechanisms that regulate EGCG levels, leaf transcriptomes were sequenced at three developmental stages (bud, second leaf, and fourth leaf) of two C. sinensis cultivars and one F-1 hybrid line #1005 that possess varying EGCG levels. Approximately 470.9 M of quality controlled-filtered sequencing paired-end reads corresponding to 47.09 G nucleotides were obtained from which 130,762 unique sequences were identified. Genes whose expression correlated with changes in EGCG levels were identified, which may be associated with EGCG content. Prediction of the potential targets of microRNAs (miRNAs), the specific subset of long noncoding RNAs (LncRNAs), and mRNA-like noncoding RNAs (mlncRNAs) indicated that miRNAs may play a role in the expression regulation of the genes involved in EGCG levels.