文献类型：期刊文献

英文题名：Development of 240 novel EST-SSRs in Eucalyptus L'Herit

作者：Zhou, Changpin[1,2] He, Xudong[3] Li, Fagen[2] Weng, Qijie[2] Yu, Xiaoli[2] Wang, Yu[2] Li, Mei[2] Shi, Jisen[3] Gan, Siming[1,2]

第一作者：周长品

通信作者：Gan, SM[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[3]Nanjing Forestry Univ, Key Lab Forest Genet & Biotechnol, Minist Educ, Nanjing 210037, Jiangsu, Peoples R China

年份：2014

卷号：33

期号：1

起止页码：221-225

外文期刊名：MOLECULAR BREEDING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000329670900018)】；

基金：This work was financially supported by grants from the Ministry of Science and Technology of China (2013AA100705, 2012CB114505 and 2011AA100202) and the National Natural Science Foundation of China (31070592). The authors are grateful to Kunming Wu and Juying Wu for plant material maintenance.

语种：英文

外文关键词：Eucalyptus; Expressed sequence tag (EST); Simple sequence repeat (SSR); Polymorphism; Transferability

摘要：Simple sequence repeat (SSR) markers derived from expressed sequence tag (EST) resources provide great potential for comparative mapping, direct gene tagging of quantitative trait loci and functional diversity studies. Here we report on the development of 240 novel EST-SSRs for the important tree genus Eucalyptus L'H,rit. Of the 240 EST-SSR loci, 218 (90.8 %) were polymorphic among 12 individuals of E. grandis Hill ex Maiden, with the number of alleles per locus (N (a)), observed heterozygosity (H (o)), expected heterozygosity (H (e)) and polymorphic information content (PIC) averaging at 5.0, 0.403, 0.598 and 0.529, respectively. High rates of cross-species/subgenus amplification were observed. The EST-SSRs developed herein would be a valuable addition of functional markers for genetics and breeding applications in a wide range of eucalypt species. The primer sequences for the 240 EST-SSRs have been deposited in the Probe database of GenBank (IDs Pr016588534-773).