文献类型：期刊文献

英文题名：The first identification of genomic loci in plants associated with resistance to galling insects: a case study in Eucalyptus L'Her. (Myrtaceae)

作者：Zhang, Miaomiao[1,2,3] Zhou, Changpin[1,2] Song, Zhijiao[1,2,4] Weng, Qijie[2] Li, Mei[2] Ji, Hongxia[2] Mo, Xiaoyong[3] Huang, Huanhua[5] Lu, Wanhong[6] Luo, Jianzhong[6] Li, Fagen[1,2] Gan, Siming[1,2]

第一作者：Zhang, Miaomiao

通信作者：Li, FG[1];Gan, SM[1];Li, FG[2];Gan, SM[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab State Forestry Adm Trop Forestry Res, Guangzhou 510520, Guangdong, Peoples R China;[3]South China Agr Univ, Coll Forestry, 284 Block,Wushan St, Guangzhou 510642, Guangdong, Peoples R China;[4]Baoshan Univ, Yuanzheng Rd, Baoshan 678000, Peoples R China;[5]Guangdong Acad Forestry, Guangzhou 510520, Guangdong, Peoples R China;[6]China Eucalypt Res Ctr, Zhanjiang 524022, Peoples R China

年份：2018

卷号：8

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000461715500001)】；

基金：We would like to thank Yonghuai Huang and Yan Lin for assistance with leaf sampling as well as Xiaoli Yu for help in the laboratory. We also thank Simon G Southerton for his critical reading and valuable comments on the manuscript. This work was financially supported by the Department of Science and Technology of Guangxi Zhuang Autonomous Region, China (Guike AA17204065-6), the National Natural Science Foundation of China (31270702) and Guangdong Provincial Department of Science and Technology (2014B020202013).

语种：英文

摘要：Genomic loci related with resistance to gall-inducing insects have not been identified in any plants. Here, association mapping was used to identify molecular markers for resistance to the gall wasp Leptocybe invasa in two Eucalyptus species. A total of 86 simple sequence repeats (SSR) markers were screened out from 839 SSRs and used for association mapping in E. grandis. By applying the mixed linear model, seven markers were identified to be associated significantly (P <= 0.05) with the gall wasp resistance in E. grandis, including two validated with a correction of permutation test (P <= 0.008). The proportion of the variance in resistance explained by a significant marker ranged from 3.3% to 37.8%. Four out of the seven significant associations in E. grandis were verified and also validated (P <= 0.073 in a permutation test) in E. tereticornis, with the variation explained ranging from 24.3% to 48.5%. Favourable alleles with positive effect were also mined from the significant markers in both species. These results provide insight into the genetic control of gall wasp resistance in plants and have great potential for marker-assisted selection for resistance to L. invasa in the important tree genus Eucalyptus.