文献类型：期刊文献

英文题名：UV-mediated synthesis of salecan and sodium alginate-based double network hydrogel for cellular and histocompatibility research

作者：Hu, Xinyu[1,2,3,4,5] Yan, Bowen[1,2,3,4,5] Xie, Pujun[1,2,3,4,5] Li, Wenjun[1,2,3,4,5] Deng, Yejun[1,2,3,4,5] Zhang, Meng[1,2,3,4,5,6] Yan, Linlin[1,2,3,4,5] Xu, Shichao[7]

第一作者：Hu, Xinyu;胡新宇

通信作者：Hu, XY[1];Yan, LL[1];Xu, SC[2]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, State Key Lab Dev & Utilizat Forest Food Resources, Nanjing 210042, Peoples R China;[2]Key Lab Biomass Energy & Mat Jiangsu Prov, Nanjing 210042, Peoples R China;[3]Natl Forestry & Grassland Adm, Key Lab Chem Engn Forest Prod, Nanjing 210042, Peoples R China;[4]Int Innovat Ctr Forest Chem & Mat, Nanjing 210042, Peoples R China;[5]Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Nanjing 210042, Peoples R China;[6]Yancheng Teachers Univ, Sch Wetlands, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224002, Peoples R China;[7]Linyi Univ, Sch Chem & Chem Engn, Linyi 276000, Peoples R China

年份：2026

卷号：337

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20255019695010);Scopus(收录号：2-s2.0-105024304807);WOS:【SCI-EXPANDED(收录号:WOS:001642089400001)】；

基金：This work was supported by the National Key Research and Devel-opment Program of China under the Grant 2024YFD2201302.

语种：英文

外文关键词：Salecan and sodium alginate-based double network hydrogel; UV-initiated polymerization and ionic crosslinking methods; Cellular and histocompatibility research

摘要：Polysaccharide-based hydrogels are promising biomaterials due to their good biocompatibility and ability to mimic natural extracellular matrices. Salecan is a new type of salt-tolerant strain fermented polysaccharide with excellent physicochemical and biological properties. Here, an advanced double network hydrogel based on salecan and sodium alginate was prepared by UV-initiated polymerization and ionic crosslinking methods. The structural stability of hydrogel was maintained mainly by the dynamic non-covalent interactions including the coordinate bonds between molybdenum ions and the functional groups of polysaccharides and the multiple hydrogen bonds among the first and second networks. The storage modulus thus kept at a high level throughout the whole frequency region, and the hydrogel also exhibited high compressive modulus and fracture strain. Surprisingly, the moderate water uptake and interconnected porous microstructure of double network hydrogel provided an ideal environment for cell culture. The results of cellular response analysis, including cytotoxicity, cell proliferation, and Live/Dead assay, showed that the hydrogel had excellent cytocompatibility. The cell density could be well regulated by the pore size and size distribution. In vivo H&E staining result indicated that the implanted hydrogel did not trigger hemolytic reactions or organ toxicity and exhibited favorable histocompatibility. The result of degradation experiment demonstrated that the hydrogel was stable during long-term use. The controllable hydrogel degradation and its subsequent separation from cells and tissues had no adverse impacts. In summary, the present study opened up a new path for the design and synthesis of polysaccharidebased hydrogel with great potential for cellular and histocompatibility research.