文献类型：期刊文献

中文题名：核桃仁内种皮中缩合单宁多聚体的分离及其抗氧化活性分析

英文题名：Isolation and antioxidant activity analysis of condensed tannin polymers in walnut pellicle

作者：崔茂凯[1,2] 黄海涛[3] 李庆杨[1] 沈丹玉[1] 肖鹏飞[3] 刘毅华[1]

第一作者：崔茂凯

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400;[2]南京林业大学风景园林学院,南京210037;[3]浙江农林大学暨阳学院生物与环境学院,浙江绍兴312000

年份：2025

卷号：50

期号：4

起止页码：60-64

中文期刊名：中国油脂

外文期刊名：China Oils and Fats

收录：;北大核心:【北大核心2023】；

基金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2019QD002)。

语种：中文

中文关键词：核桃;内种皮;缩合单宁;多聚体;抗氧化活性

外文关键词：walnut;pellicle;condensed tannins;polymer;antioxidant activity

分类号：TS222.1;TS229

摘要：为给核桃资源的高值化利用提供参考,以核桃仁内种皮为原料,通过70%甲醇溶液(含0.5%冰乙酸)萃取,Sephadex LH-20葡聚糖凝胶层析柱分离,经不同体积分数的甲醇溶液梯度洗脱得到13个核桃仁内种皮缩合单宁多聚体分离物(F1~F13),以V_(C)为标样,对这13个分离物的体外抗氧化活性进行分析。结果表明:采用甲醇溶液(体积分数40%~75%)梯度洗脱方法可从核桃仁内种皮中分离出缩合单宁二聚体(DP2)至七聚体(DP7);DP2和缩合单宁三聚体(DP3)的洗脱甲醇溶液体积分数在40%~60%,缩合单宁四聚体(DP4)和其他3种高聚体的洗脱甲醇溶液体积分数在60%~75%;优化洗脱操作后DP2和DP3可被高效分离,DP2洗脱率超过80%,其中F2、F3和F4中得到DP2,总含量超过1.40 mg/g,F5和F6中得到DP3,含量分别为0.66 mg/g和0.59 mg/g;F1~F13都具有良好的抗氧化活性,其中以DP2为主的F4抗氧化活性最高,铁离子还原能力(FRAP)和DPPH自由基清除能力分别为(17.22±0.68)mg/g和(24.36±0.73)mg/g。综上,采用梯度洗脱方法可实现缩合单宁多聚体的洗脱分离,可从核桃仁内种皮中分离出DP2和DP3。
To provide a reference for the high-value utilization of walnut resources,walnut pellicle was used as raw material and extracted with a 70%methanol solution(containing 0.5%acetic acid).The extract was separated with a Sephadex LH-20 dextran gel column and subjected to gradient elution with different volume fractions of methanol solution to obtain 13 fractions of condensed tannin polymers(F1-F13).The in vitro antioxidant activity of these 13 fractions were analyzed using vitamin C as the standard sample.The results indicated that the gradient elution method with methanol solution(volume fraction 40%-75%)could successfully separate condensed tannin dimers(DP2)to heptamers(DP7)from walnut pellicle.The elution volume fractions for condensed DP2 and trimers(DP3)ranged from 40%to 60%,while the elution volume fractions for tetramers(DP4)and three other higher polymers were between 60%and 75%.After optimizing the elution process,efficient separation of DP2 and DP3 was achieved,with the elution rate of DP2 exceeding 80%.DP2 was isolated from elution fractions F2,F3,and F4,with a tatal content exceeding 1.40 mg/g,while DP3 was obtained from fractions F5 and F6,with contents of 0.66 mg/g and 0.59 mg/g,respectively.All fractions exhibited good antioxidant activity,with F4,primarily containing DP2,showing the highest antioxidant activity,measured by ferric ion reducing ant ioxidant power(FRAP)and DPPH methods as(17.22±0.68)mg/g and(24.36±0.73)mg/g,respectively.In conclusion,the gradient elution method enables the effective separation of condensed tannin polymers,allowing for the isolation of DP2 and DP3 from walnut pellicle.