文献类型：期刊文献

中文题名：桑叶总多酚筛选和分离纯化工艺研究

英文题名：Screening,Separation and Purification of Mulberry Leaf Polyphenols

作者：周昊[1,2,3,4,5] 李文君[2,3,4,5] 张俊[1] 张宇思[2,3,4,5] 颜新培[1] 王成章[1,2,3,4,5]

第一作者：周昊

通信作者：Yan, Xin-Pei

机构：[1]湖南省蚕桑科学研究所;[2]中国林业科学研究院林产化学工业研究所;[3]生物质化学利用国家工程实验室;[4]国家林业局林产化学工程重点开放性实验室;[5]江苏省生物质能源与材料重点实验室

年份：2015

卷号：35

期号：6

起止页码：133-140

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;EI(收录号：20161202132631);Scopus(收录号：2-s2.0-84960950933);北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：“十二五”国家科技支撑计划资助项目(2012BAD36B07)

语种：中文

中文关键词：桑叶总多酚;超声波微波协同提取;响应面优化;大孔树脂纯化

外文关键词：mulberry leaf polyphenols; ultrasonic-microwave combined extraction; response surface optimization; maeroporousabsorbent resins

分类号：TQ35

摘要：采用超声波和微波协同提取桑叶总多酚,Folin-Ciocalte法分析不同品种桑叶的总多酚含量,运用单因素试验和响应面法优化提取工艺,并考察不同种类大孔树脂对桑叶总多酚的纯化效果。结果表明,18个品种桑叶中大墨斗的总多酚质量分数最高,达到2.072%;灃24×苗33总多酚质量分数最低,仅为1.035%。湖桑87桑叶总多酚最佳提取工艺为:1 g桑叶粉末,液料比20∶1(mL∶g),提取时间93 s,微波功率326 W,超声波功率304 W,粗提物得率为14.7%,纯度为10.87%。筛选得到的较适合桑叶总多酚纯化的H103树脂的吸附量为15.57 mg/g,解吸率为88.67%,且较短时间内达到吸附平衡与解吸平衡。上样时吸附柱体积40 mL,上样量15 g粗提物,桑叶总多酚的最佳质量浓度为3 g/L,此时最佳洗脱条件为乙醇体积分数60%、洗脱流速2 mL/min、洗脱体积80 mL,此条件下,桑叶总多酚纯度由10.87%提高到65.45%,纯化过程中桑叶总多酚得率为84.7%。
Ultrasonic-microwave combined technology was used to extract polyphenols from the mulberry leaves and Folin-Ciocalte method was applied to analyze the content of polyphenols in different types and parts of mulberry leaves. The single factor and response surface methodology were successfully used to optimize the extraction conditions. Furthermore, the purification effects of different macroporous resins on the polyphenols were analyzed. The results showed that the highest content of polyphenols was 2. 072 % from the type of Damodou, and the polyphenols content of Feng 24 Х Miao 33 was the lowest （ 1. 035 % ） among eighteen varieties of mulberry leave. The optimal extraction conditions were 1 g Hu-sang87, liquid to solid ratio 20 ： 1 （ mL ： g）, the extraction time 93 s, microwave power 326 W, ultrasonic power 304 W. Meanwhile, the yield of crude extracts was 1. 598 % and the purity was 10.87 %. The most suitable resin for this purification was H103 with adsorbing capacity of 15.57 mg/g, desorption ratio of 88.67 % and short adsorption and desorption equilibrium time. The best adsorbing condition of polyphenols sample solution were adsorption column volume 40 mL, loading quantity of 15 g crude extract, mass concentration 3 g/L and the best elution conditions were ethanol volume fraction 60 % , flow rate 2 mL/min, elution volume 80 mL. Then, the purity of polyphenol increased from 10.87 % to 65.45 % under these conditions,and in the process of purification, the total yield was 84.7 %.