文献类型：期刊文献

英文题名：Xylem Transcriptome Analysis in Contrasting Wood Phenotypes of Eucalyptus urophylla x tereticornis Hybrids

作者：Zhu, Xianliang[1] He, Jiayue[1] Zhou, Changpin[1] Weng, Qijie[1] Chen, Shengkan[2,3] Bush, David[4] Li, Fagen[1]

第一作者：Zhu, Xianliang

通信作者：Li, FG[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab Natl Forestry & Grassland Adm Trop Forest, Guangzhou 510520, Peoples R China;[2]Guangxi Forestry Res Inst, Guangxi Key Lab Super Timber Trees Resource Culti, Nanning 530002, Peoples R China;[3]Guangxi Forestry Res Inst, Key Lab Cent South Fast Growing Timber Cultivat F, Nanning 530002, Peoples R China;[4]CSIRO, Australian Tree Seed Ctr, GPO Box 1600, Canberra, ACT 2601, Australia

年份：2022

卷号：13

期号：7

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000832333100001)】；

基金：This work was financially supported by the Fundamental Research Funds of ChineseAcademy of Forestry (CAFYBB2021ZA001), Guangdong Natural Science Foundation (2020A1515010974), and Ministry of Science and Technology of China (2016YFD0600101).

语种：英文

外文关键词：Eucalyptus; RNA splicing; transcription factors; wood formation; differentially expressed gene

摘要：An investigation of the effects of two important post-transcriptional regulatory mechanisms, gene transcription and alternative splicing (AS), on the wood formation of Eucalyptus urophylla x tereticornis, an economic tree species widely planted in southern China, was carried out. We performed RNA-seq on E. urophylla x tereticornis hybrids with highly contrasting wood basic density (BD), cellulose content (CC), hemicellulose content (HC), and lignin content (LC). Signals of strong differentially expressed genes (DEGs) and differentially spliced genes (DSGs) were detected in all four groups of wood properties, suggesting that gene transcription and selective splicing may have important regulatory roles in wood properties. We found that there was little overlap between DEGs and DSGs in groups of the same trait. Furthermore, the key DEGs and DSGs that were detected simultaneously in the four groups tended to be enriched in different Gene Ontology terms, Kyoto Encyclopedia of Genes and Genomes pathways, and transcription factors. These results implied that regulation of gene transcription and AS is controlled by independent regulatory systems in wood formation. Lastly, we detected transcript levels of known wood biosynthetic genes and found that 79 genes encoding mainly enzymes or proteins such as UGT, LAC, CAD, and CESA may be involved in the positive or negative regulation of wood properties. This study reveals potential molecular mechanisms that may regulate wood formation and will contribute to the genetic improvement of Eucalyptus.