文献类型：期刊文献

英文题名：Integrative transcriptomic and metabolomic analyses unveil tanshinone biosynthesis in Salvia miltiorrhiza root under N starvation stress

作者：Lu, Li-Lan[1,2] Zhang, Yu-Xiu[1] Yang, Yan-Fang[3]

第一作者：Lu, Li-Lan

通信作者：Lu, LL[1];Lu, LL[2]

机构：[1]Chinese Acad Med Sci & Peking Union Med Coll, Hainan Branch Inst Med Plant Dev, Hainan Prov Key Lab Resources Conservat & Dev Sout, Haikou, Peoples R China;[2]Chinese Acad Trop Agr Sci, Coconut Res Inst, Hainan Key Lab Trop Oil Crops Biol, Wenchang, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding, Beijing, Peoples R China

年份：2022

卷号：17

期号：8

外文期刊名：PLOS ONE

收录：;Scopus(收录号：2-s2.0-85136930219);WOS:【SCI-EXPANDED(收录号:WOS:000950926900001)】；

基金：The authors thank Dr. Shaojun Jie (The Research Institute of Forestry) for their chart-making suggestions. We also thank Oebiotech (www.oebiotech.com) for transcriptome sequencing and data processing, and we thank Editage for editing language.

语种：英文

摘要：Salvia miltiorrhiza is a model plant for Chinese herbal medicine with significant pharmacologic effects due to its tanshinone components. Our previous study indicated that nitrogen starvation stress increased its tanshinone content. However, the molecular mechanism of this low nitrogen-induced tanshinone biosynthesis is still unclear. Thus, this study aimed to elucidate the molecular mechanism of tanshinone biosynthesis in S. miltiorrhiza under different N conditions [N-free (N0), low-N (Nl), and full-N (Nf, as control) conditions] by using transcriptome and metabolome analyses. Our results showed 3,437 and 2,274 differentially expressed unigenes between N0 and Nf as well as Nl and Nf root samples, respectively. N starvation (N0 and Nl) promoted the expression of the genes involved in the MVA and MEP pathway of tanshinone and terpenoid backbone biosynthesis. Gene ontology and KEGG analyses revealed that terpenoid backbone biosynthesis, hormone signal transduction, and phenylpropanoid biosynthesis were promoted under N starvation conditions, whereas starch and sucrose metabolisms, nitrogen and phosphorus metabolisms, as well as membrane development were inhibited. Furthermore, metabolome analysis showed that metabolite compounds and biosynthesis of secondary metabolites were upregulated. This study provided a novel insight into the molecular mechanisms of tanshinone production in S. miltiorrhiza in response to nitrogen stress.