文献类型：期刊文献

中文题名：尾细桉生长和木材密度关联SNP挖掘与候选基因定位

英文题名：Association of SNP loci and candidate genes for growth and wood density in Eucalyptus urophylla × E. tereticornis

作者：朱显亮[1] 周长品[1] 贾翠蓉[1] 翁启杰[1] 李发根[1]

第一作者：朱显亮

机构：[1]中国林业科学研究院热带林业研究所,热带林业研究国家林业和草原局重点实验室,广东广州510520

年份：2021

卷号：45

期号：4

起止页码：143-150

中文期刊名：南京林业大学学报：自然科学版

外文期刊名：Journal of Nanjing Forestry University:Natural Sciences Edition

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：广东省自然科学基金项目(2020A1515010974);中国林科院基本科研业务费专项(CAFYBB2017MA006)。

语种：中文

中文关键词：尾细桉;测序分型;单核苷酸多态性(SNP);表型性状;关联分析;候选基因

外文关键词：Eucalyptus urophylla×E.tereticornis;genotyping by sequencing;single nucleotide polymorphism(SNP);phenotypic character;association analysis;candidate genes

分类号：S781

摘要：【目的】生长和木材基本密度是桉树的重要经济性状,挖掘其候选功能基因可为桉树遗传改良提供参考。【方法】以尾叶桉(Eucalyptus urophylla)和细叶桉(Eucalyptustereticornis) F1全同胞子代试验林为研究对象,测定其8年生树高、胸径和木材基本密度,开展表型遗传分析。筛选极端表型个体,利用测序分型(GBS)开发SNP标记进行关联分析。挖掘与树高、胸径和木材基本密度关联的SNP位点,并进行候选基因初步定位。【结果】尾细桉F1子代树高、胸径与木材基本密度间的表型变异系数为7.51%~26.19%,生长性状与木材基本密度显著正相关。利用GBS获得了覆盖全基因组的15 185个SNP位点,关联分析共鉴定111个与生长和木材基本密度显著关联的SNP,其中2号染色体上检测到强烈的生长性状关联信号。定位40个与生长和木材基本密度相关候选基因,共富集在52个GO terms,基因功能注释分析表明其功能主要与植物抗逆性、生物与非生物胁迫、转录因子家族等相关。【结论】本研究获得了一批与尾细桉生长和材性性状关联的SNP位点和候选基因,并进行了树高、胸径及木材基本密度候选基因初步定位,挖掘的与抗逆性相关的基因可能在树木的生长和木材形成中发挥重要作用。
【Objective】Growth and basic wood density are important economic traits of Eucalyptus;thus,searching for candidate genes will facilitate the genetic improvement in this genus.【Method】The interspecific full-sib family of E.urophylla and E.tereticornis was employed in this study.In addition,the correlation between the growth traits(height,dia-meter)and basic wood density of eight-year-old trees was investigated.The extreme phenotypes for each trait were screened for genotyping by sequencing(GBS)and developing SNP loci.Subsequently,association analyses of phenotypes and SNP loci were performed.Finally,SNP loci associated with the growth traits and basic wood density of E.urophylla×E.tereticornis were identified;candidate genes for these related traits were also identified.【Result】①The phenotypic coefficient of variation ranged from 7.51%to 26.19%among the values of height,diameter,and basic wood density,while a significant positive correlation between the growth traits and basic wood density was found.②A total of 15185 whole-genome SNP loci were developed,and a total of 111 SNPs were identified as being significantly associated with growth and basic wood density;among which,strong association signals for diameter were detected on chromosome No.2.③In addition,40 candidate genes related to the growth and basic wood density were identified,which were enriched in 52 GO terms.Gene annotation indicated that the candidate genes were mainly involved in plant stress resis-tance,biotic and abiotic stress,and transcription factor families.【Conclusion】In this study,a new set of SNP loci rela-ted to the growth traits and wood properties of E.urophylla×E.tereticornis were obtained,and dozens of candidate genes were identified.Our results suggest that genes related to stress resistance may play an important role in tree growth and wood formation.This information provides valuable genetic resources for research on Eucalyptus breeding.