文献类型：期刊文献

中文题名：响应曲面法优化高纯度油橄榄叶橄榄苦苷酶解工艺

英文题名：Optimization of Enzymatic Hydrolysis of High Purity Oleuropein from Olea europaea( Olive) Leaf Extract by Response Surface Methodology

作者：原姣姣[1,2,3,4] 王成章[1,2,3,4,5] 叶建中[1,2,3,4] 陈虹霞[1,2,3,4] 张宇思[1,2,3,4] 陶冉[1,2,3,4] 周昊[1,2,3,4,5] 刘玉红[6]

第一作者：原姣姣

机构：[1]中国林业科学研究院林产化学工业研究所;[2]生物质化学利用国家工程实验室;[3]国家林业局林产化学工程重点开放性实验室;[4]江苏省生物质能源与材料重点实验室;[5]中国林业科学研究院林业新技术研究所;[6]陇南市翔宇油橄榄开发有限公司

年份：2015

卷号：27

期号：9

起止页码：1643-1650

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国际科技合作专项(2014DFR31300);甘肃省科技重大专项(143NKDK025)

语种：中文

中文关键词：油橄榄叶;羟基酪醇;橄榄苦苷;酶解;半纤维素酶

外文关键词：Olea europea Leaf; hydroxytyrosoi; oleuropein; enzymatic hydrolysis; hemiceUulase

分类号：Q946

摘要：本文筛选了各种酶系对橄榄苦苷的水解效果,并探讨了p H、温度、时间、酶底物比四个因素对半纤维素酶水解橄榄苦苷效率的影响。在此基础上,采用BBD实验设计方案(Box-Behnken Design)进行三因素三水平响应面分析,考察p H、温度、酶底物比及三者交互作用对油橄榄叶橄榄苦苷降解为羟基酪醇的酶解影响,并对其工艺优化。结果表明,半纤维素酶对橄榄苦苷的降解效果最佳,优于β-葡萄糖苷酶的降解效果。橄榄苦苷酶解制备羟基酪醇的最佳工艺为p H 5、温度55℃、酶底物比为1∶9.1、时间6 h,得到羟基酪醇含量为(11.31±0.15)%,橄榄苦苷的降解率为98.54%。这将为油橄榄叶橄榄苦苷酶解制备羟基酪醇的工业化生产提供有效借鉴。
The selective enzyme was screened from the 10 enzymes with high degradation rate of oleuropein （OE）. The process of single factor （pH,tempemtum,time,the ratio of enzyme quantity and substrate） and a Box-Behnken Design on enzymatic hydrolysis of 81.04% OE olive leaf extract were studied. It was suggested that preparation of hydroxytyrosol from enzymatic hydrolysis of OE in the olive leaves was feasible. The result showed that the performance of hydrolysis treatments was best using hemiceUulasc as a bio-catalyst,and the high purity of OE in olive extract was beneficial to bio- trans^orm OE into hydroxytyresol （HT）. The optimal enzymatic conditions for achieving maximal yield of HT content ob- tained by the regression were as follows：pH 5 ,temperature 55 ℃ and the ratio of enzyme quantity and substrate 1： 9.1. The experimental result was （ 11.31 ± 0. 15 ） %, and the degradation rate of OE was 98.54%. The presented results will provide effective foundation for hydroxytyrosol preparation from oleurpein in olive leaf extract.