文献类型：期刊文献

中文题名：曼地亚红豆杉NPR1基因家族鉴定及表达模式分析

英文题名：Identification and expression pattern analysis of NPR1 genes in Taxus×media

作者：杜雨晴[1] 袁菊红[2] 王旭[3] 张恺恺[1] 陈段芬[3] 邱德有[1] 杨艳芳[1]

第一作者：杜雨晴

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,北京100091;[2]江西财经大学艺术学院风景园林与植物资源研究所,江西南昌330032;[3]河北农业大学园艺学院,河北保定071001

年份：2024

卷号：55

期号：1

起止页码：240-248

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金资助项目(32371907)。

语种：中文

中文关键词：红豆杉;曼地亚红豆杉;NPR1家族;生物信息学;紫杉醇;表达分析

外文关键词：Taxus wallichiana var.chinensis(Pilger)Florin;Taxus×media Rehder;NPR1 family;bioinformatics;taxol;expression analysis

分类号：R282.12

摘要：目的通过鉴定曼地亚红豆杉Taxus×media中病程相关基因非表达子1(non-expressor of pathogenesis-related genes1,NPR1)家族基因,分析并比较其蛋白结构以及基因表达等差异,为利用NPR1基因提高红豆杉紫杉醇含量奠定理论基础。方法利用本地化blast对曼地亚红豆杉NPR1基因进行鉴定,通过CD-search、MEGA X、DNAMAN以及MEME在线网站等生物信息学技术进行保守结构域、进化树构建、多序列比对以及基序分析,利用blast比对转录组数据,预测所获得的NPR1基因在不同组织以及激素和低温处理后的表达模式。结果在曼地亚红豆杉中共鉴定出3个NPR1家族基因,命名为TmNPR1~TmNPR3。研究发现,3个NPR1家族成员均含有BTB_POZ保守结构域,并分成2个亚家族。TmNPR1/2与AtNPR3/4聚在一起,TmNPR3与AtNPR5/6亲缘关系较近。Tm NPR1/2与AtNPR3/4相似,均含有EAR基序(VDLNETP)。表达模式分析发现3个NPR1基因在不同组织中的表达有所差异,TmNPR1/2在针叶组织中的表达水平最高,TmNPR1响应低温胁迫,且在茉莉酸甲酯和冠菌素激素处理曼地亚红豆杉细胞系中表达量最高。结论曼地亚红豆杉NPR1家族的3个成员在序列和结构上存在保守性,但也在红豆杉根和针叶的发育以及在低温等不同胁迫和茉莉酸甲酯信号途径中扮演了不同的角色,推测具有不同的功能,为揭示TmNPR1s基因功能和利用分子生物学技术提高红豆杉紫杉醇产量提供理论依据。
Objective To provide a theoretical basis for increasing taxol content of Hongdoushan(Taxus wallichiana var.chinensis)by non-expressor of pathogenesis-related genes 1(NPR1)gene through identifying the family gene of NPR1 in T.×media and analyzing and comparing its differences in protein structure and gene expression.Methods Localized-blast was used to identify the NPR1 gene.Bioinformatics techniques,such as CD-search,MEGA X,DNAMAN,and MEME online websites were used to construct the conservative domain,phylogenetic tree construction,multiple sequence alignment,and motif analysis.The transcriptome data were compared by blast to predict the expression patterns of the obtained NPR1 gene in different tissues and after hormone and low-temperature treatments.Results A total of three NPR1 genes were identified in T.×media,which were named TmNPR1-3.It was found that all the three NPR1 family members contained BTB_POZ conservative domains and were divided into two subfamilies.Moreover,TmNPR1/2 and AtNPR3/4 were clustered together,and TmNPR3 and AtNPR5/6 were closely related.TmNPR1/2 was similar to AtNPR3/4 in which both of them contained an EAR motif(VDLNETP).The expression pattern analysis showed that the expression of the three NPR1 genes was different in different tissues. It is worth noting that the expression level of TmNPR1/2 was the highest in needles, and TmNPR1 was the highest in methyl jasmonate (MeJA) and coronatine-treated T. × mediacell lines in response to low temperature stress. Conclusion The three members of the NPR1 family in T. × media were conserved in sequence and structure, and they may play different roles in the development of roots and needles as well as in hormonal signals and abiotic stresses, such as MeJA signaling pathways and low temperature, which was speculated to have different functions. This study provides a theoretical basis for revealing the function of TmNPR1s genes and improving the taxol content in T. wallichiana var.chinensis by molecular biology technology.