文献类型：期刊文献

中文题名：降解没食子酸制备焦性没食子酸的菌种筛选及工艺条件

英文题名：Screening Strains and Conditions for the Degradation of Gallic Acid to Prepare Pyrogallol

作者：李文君[1] 王成章[1] 闵凡芹[1] 陶冉[1] 周昊[1,2]

第一作者：李文君

机构：[1]中国林业科学研究院林产化学工业研究所;[2]中国林业科学研究院林业新技术研究所

年份：2015

卷号：32

期号：1

起止页码：52-58

中文期刊名：精细化工

外文期刊名：Fine Chemicals

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家高科技研究发展计划(863项目2014AA021802);国际合作项目(中俄项目2014DFR31300)~~

语种：中文

中文关键词：焦性没食子酸;底物诱导;产气肠杆菌;响应面;模型修正;生物工程

外文关键词：pyrogallol; substrate induction test; Enterobacter aerogenes; response surface; model modification; biological engineering

分类号：Q815

摘要：通过研究不同微生物菌属——肠杆菌属(Enterobacer spp.)、柠檬酸杆菌属(Citrobacter spp.)菌株在CDM培养基中经底物诱导降解没食子酸生产焦性没食子酸,筛选出产气肠杆菌(Enterobacer aerogenes)为焦性没食子酸高产菌种。对发酵温度、底物浓度、初始发酵液p H等单因素对焦性没食子酸收率的影响进行了分析,并采用Box-Behnken实验设计进行三因素三水平的响应面分析及模型修正,确定了产气肠杆菌(Enterobacer aerogenes)降解没食子酸的最佳发酵条件。结果表明,在发酵温度为32℃、底物质量分数为0.32%、初始发酵液p H=6.0时,焦性没食子酸收率最大为77.86%,优于同等条件下的化学法及其他复杂的生物法,为微生物法降解没食子酸生产焦性没食子酸的规模化生产提供参考。
The strains belonging to Enterobacter spp.and Citrobacter spp.were tested in their degradation of gallic acid to produce pyrogallol in the CDM medium through the substrate induction test,and Enterobacter aerogenes was screened as the stable high-yielding strain for pyrogallol.The influence factors affecting the yield of pyrogallol,including fermentation temperature,substrate concentration,initial p H of fermentation medium,were explored.The Box-Behnken experimental analysis was adopted to design a response surface methodology based on 3 factors and 3 levels and model modification in order to determine the optimum fermentation conditions for the degradation of gallic acid with Enterobacter aerogenes.The verified experiment results show that the yield of pyrogallol could reach 77.86% when the fermentation temperature was 32 ℃,substrate concentration was0.32%,and p H of the initial fermentation medium was 6.0,and this yield was better than that obtained by the chemical method or other biological method under similar conditions.This research laid the foundation for the large-scale production of pyrogallol by biological degradation of gallic acid.